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Information on EC 1.14.11.60 - scopoletin 8-hydroxylase for references in articles please use BRENDA:EC1.14.11.60
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EC Tree
IUBMB Comments Requires iron(II) and ascorbate. A protein involved in biosynthesis of iron(III)-chelating coumarins in higher plants.
The expected taxonomic range for this enzyme is: Arabidopsis thaliana
Synonyms
At3g12900, At3g12900 protein, S8H,
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At3g12900
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At3g12900 protein
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S8H
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gene name
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scopoletin + 2-oxoglutarate + O2 = fraxetin + succinate + CO2
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scopoletin,2-oxoglutarate:oxygen oxidoreductase (8-hydroxylating)
Requires iron(II) and ascorbate. A protein involved in biosynthesis of iron(III)-chelating coumarins in higher plants.
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scopoletin + 2-oxoglutarate + O2
fraxetin + succinate + CO2
scopoletin + 2-oxoglutarate + O2
fraxetin + succinate + CO2
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scopoletin + 2-oxoglutarate + O2
fraxetin + succinate + CO2
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scopoletin + 2-oxoglutarate + O2
fraxetin + succinate + CO2
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the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants
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scopoletin + 2-oxoglutarate + O2
fraxetin + succinate + CO2
the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis
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scopoletin + 2-oxoglutarate + O2
fraxetin + succinate + CO2
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the enzyme participates in Fe acquisition by mediating the biosynthesis of fraxetin (7,8-dihydroxy-6-methoxycoumarin), a coumarin derived from the scopoletin pathway
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scopoletin + 2-oxoglutarate + O2
fraxetin + succinate + CO2
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scopoletin + 2-oxoglutarate + O2
fraxetin + succinate + CO2
the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis
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scopoletin + 2-oxoglutarate + O2
fraxetin + succinate + CO2
scopoletin + 2-oxoglutarate + O2
fraxetin + succinate + CO2
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the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants
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scopoletin + 2-oxoglutarate + O2
fraxetin + succinate + CO2
Q9LE86
the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis
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scopoletin + 2-oxoglutarate + O2
fraxetin + succinate + CO2
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the enzyme participates in Fe acquisition by mediating the biosynthesis of fraxetin (7,8-dihydroxy-6-methoxycoumarin), a coumarin derived from the scopoletin pathway
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?
scopoletin + 2-oxoglutarate + O2
fraxetin + succinate + CO2
Q9LE86
the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis
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Fe2+
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enzyme efficiency is best in the presence of 0.05 mM Fe2+
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Fe2+
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concentrations above 0.05 mM significantly reduce the enzyme activity, leading to decreased fraxetin synthesis
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0.011
scopoletin
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pH 8.0, 31.5°C
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1.73
scopoletin
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pH 8.0, 31.5°C
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157.3
scopoletin
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pH 8.0, 31.5°C
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SwissProt
brenda
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SwissProt
brenda
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brenda
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expressed most strongly in the root epidermal layer under iron-deficient conditions
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brenda
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malfunction
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mutants defective in the expression of S8H show increased sensitivity to growth on pH 7.0 media supplemented with an immobile source of Fe and reduced secretion of fraxetin. Transgenic lines overexpressing S8H exhibit an opposite phenotype. Supplementing the media containing immobile Fe with fraxetin partially rescues the s8h mutants
physiological function
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the enzyme participates in Fe acquisition by mediating the biosynthesis of fraxetin (7,8-dihydroxy-6-methoxycoumarin), a coumarin derived from the scopoletin pathway. Fraxetin secretion is a decisive factor for calcicole-calcifuge behavior (i.e. the ability/inability to thrive on alkaline soils) of plants
metabolism
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the enzyme is involved in coumarin biosynthesis and is part of mechanisms used by plants to assimilate iron
metabolism
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the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants. Under iron-deficiency conditions, a branch of the phenylpropanoid pathway consisting of feruloyl-CoA 6'-hydroxylase 1, scopoletin 8-hydroxylase, and CYP82C4 is strongly induced, thus leading to the synthesis of scopoletin and its conversion into redox-active fraxetin and sideretin. In the rhizosphere, the redox-active coumarins act in concert with other components of the strategy I iron acquisition machinery by solubilizing and reducing iron from sparingly available sources
metabolism
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the enzyme participates in Fe acquisition by mediating the biosynthesis of fraxetin (7,8-dihydroxy-6-methoxycoumarin), a coumarin derived from the scopoletin pathway. Fraxetin secretion is a decisive factor for calcicole-calcifuge behavior (i.e. the ability/inability to thrive on alkaline soils) of plants
metabolism
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the enzyme is involved in coumarin biosynthesis and is part of mechanisms used by plants to assimilate iron
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S8H_ARATH
357
0
39716
Swiss-Prot
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expression of S8H protein in Nicotiana benthamiana leaves; heterologous expression in Escherichia coli
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heterologous-pathway reconstitution in Nicotiana benthamiana
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iron-deficiency-dependent upregulation. The iron-deficiency-dependent upregulation is mainly confined to roots
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the enzyme is highly induced in roots of Fe-deficient plants both at the transcript and protein levels
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Siwinska, J.; Siatkowska, K.; Olry, A.; Grosjean, J.; Hehn, A.; Bourgaud, F.; Meharg, A.A.; Carey, M.; Lojkowska, E.; Ihnatowicz, A.
Scopoletin 8-hydroxylase a novel enzyme involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis
J. Exp. Bot.
69
1735-1748
2018
Arabidopsis thaliana, Arabidopsis thaliana (Q9LE86), Arabidopsis thaliana Col-0 (Q9LE86)
brenda
Rajniak, J.; Giehl, R.; Chang, E.; Murgia, I.; Von Wiren, N.; Sattely, E.
Biosynthesis of redox-active metabolites in response to iron deficiency in plants
Nat. Chem. Biol.
14
442-450
2018
Arabidopsis thaliana (Q9LE86)
brenda
Tsai, H.H.; Rodriguez-Celma, J.; Lan, P.; Wu, Y.C.; Velez-Bermudez, I.C.; Schmidt, W.
Scopoletin 8-hydroxylase-mediated fraxetin production is crucial for iron mobilization
Plant Physiol.
177
194-207
2018
Arabidopsis thaliana, Arabidopsis thaliana (Q9LE86)
brenda
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