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EC 4.1.99.14 Details
show Brenda entryEC number
4.1.99.14
Accepted name
spore photoproduct lyase
Reaction
(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine (in double-helical DNA) = thymidylyl-(3′→5′)-thymidylate (in double-helical DNA)
Other name(s)
SAM,
SP lyase,
SPL,
SplB,
SplG
Systematic name
spore photoproduct pyrimidine-lyase
CAS registry number
37290-70-3
Comment
This enzyme is a member of the ’AdoMet radical’ (radical SAM) family. The enzyme binds a [4Fe-4S] cluster. The cluster is coordinated by 3 cysteines and an exchangeable SAM molecule [3]. The 5′-deoxy-adenosine radical formed after electron transfer from the [4Fe-4S] cluster to the S-adenosyl-L-methionine, initiates the repair by abstracting the C-6 hydrogen of the spore photoproduct lesion. During the second part of the repair process the SAM molecule is regenerated [3].
History
created 2009, modified 2010
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
4.1.99.4 created 1981, deleted 2002 4.1.99.6 created 1989, deleted 2000 4.1.99.8 created 2000, deleted 2000 4.1.99.9 created 2000, deleted 2000 4.1.99.10 created 2000, deleted 2000
