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EC 4.1.99.1 Details
show Brenda entryEC number
4.1.99.1
Accepted name
tryptophanase
Reaction
L-tryptophan + H2O = indole + pyruvate + NH3 (overall reaction);;(1a) L-tryptophan = indole + 2-aminoprop-2-enoate;;(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous);;(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous)
Other name(s)
L-tryptophanase,
L-tryptophan indole-lyase (deaminating),
TNase
Systematic name
L-tryptophan indole-lyase (deaminating; pyruvate-forming)
CAS registry number
9024-00-4
Comment
A pyridoxal-phosphate protein, requiring K+. The enzyme cleaves a carbon-carbon bond, releasing indole and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. Also catalyses 2,3-elimination and β-replacement reactions of some indole-substituted tryptophan analogues of L-cysteine, L-serine and other 3-substituted amino acids.
History
created 1972
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
4.1.99.4 created 1981, deleted 2002 4.1.99.6 created 1989, deleted 2000 4.1.99.8 created 2000, deleted 2000 4.1.99.9 created 2000, deleted 2000 4.1.99.10 created 2000, deleted 2000
