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EC 3.2.2.28 Details

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EC number

3.2.2.28

Accepted name

double-stranded uracil-DNA glycosylase

Reaction

Specifically hydrolyses mismatched double-stranded DNA and polynucleotides, releasing free uracil

Other name(s)

Mug, double-strand uracil-DNA glycosylase, Dug, dsUDG, double-stranded DNA specific UDG, dsDNA specific UDG, UdgB (ambiguous), G:T/U mismatch-specific DNA glycosylase, UDG (ambiguous)

Systematic name

uracil-double-stranded DNA deoxyribohydrolase (uracil-releasing)

Comment

No activity on DNA containing a T/G mispair or single-stranded DNA containing either a site-specific uracil or 3,N⁴-ethenocytosine residue [2], significant role for double-stranded uracil-DNA glycosylase in mutation avoidance in non-dividing E. coli [3]. Uracil-DNA glycosylases are widespread enzymes that are found in all living organisms. Uracil-DNA glycosylase (EC 3.2.2.27) and EC 3.2.2.28 form a central part of the DNA-repair machinery since they initiate the DNA base-excision repair pathway by hydrolysing the N-glycosidic bond between uracil and the deoxyribose sugar thereby catalysing the removal of mis-incorporated uracil from DNA.

History

created 2009

EC Tree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.2 Acting on the aldehyde or oxo group of donors

1.3 Acting on the CH-CH group of donors

1.3.1 With NAD⁺ or NADP⁺ as acceptor

1.3.2 With a cytochrome as acceptor

1.3.3 With oxygen as acceptor

1.3.4 With a disulfide as acceptor

1.3.5 With a quinone or related compound as acceptor

1.3.7 With an iron-sulfur protein as acceptor

1.3.8 With a flavin as acceptor

1.3.98 With other, known, physiological acceptors

1.3.99 With unknown physiological acceptors

1.4 Acting on the CH-NH₂ group of donors

1.5 Acting on the CH-NH group of donors

1.6 Acting on NADH or NADPH

1.7 Acting on other nitrogenous compounds as donors

1.8 Acting on a sulfur group of donors

1.9 Acting on a heme group of donors

1.10 Acting on diphenols and related substances as donors

1.10.1 With NAD⁺ or NADP⁺ as acceptor

1.10.2 With a cytochrome as acceptor

1.10.3 With oxygen as acceptor

1.10.5 With a quinone or related compound as acceptor

1.10.9 With a copper protein as acceptor

1.10.99 With unknown physiological acceptors

1.11 Acting on a peroxide as acceptor

1.12 Acting on hydrogen as donor

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.15 Acting on superoxide as acceptor

1.15.1 Acting on superoxide as acceptor (only sub-subclass identified to date)

1.16 Oxidizing metal ions

1.17 Acting on CH or CH₂ groups

1.18 Acting on iron-sulfur proteins as donors

1.19 Acting on reduced flavodoxin as donor

1.20 Acting on phosphorus or arsenic in donors

1.21 Catalysing the reaction X-H + Y-H = X-Y

1.21.1 With NAD⁺ or NADP⁺ as acceptor

1.21.3 With oxygen as acceptor

1.21.4 With a disulfide as acceptor

1.21.98 With other, known, physiological acceptors

1.21.99 With unknown physiological acceptors

1.22 Acting on halogen in donors

1.23 Reducing C-O-C group as acceptor

1.97 Other oxidoreductases

1.97.1 Sole sub-subclass for oxidoreductases that do not belong in the other subclasses

1.98 Enzymes using H₂ as reductant (deleted subclass)

1.99 Other enzymes using O₂ as oxidant (deleted subclass)

3.1 Acting on ester bonds

3.2 Glycosylases

3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

3.2.2 Hydrolysing N-glycosyl compounds

3.2.2.1 purine nucleosidase

3.2.2.2 inosine nucleosidase

3.2.2.3 uridine nucleosidase

3.2.2.4 AMP nucleosidase

3.2.2.5 NAD⁺ glycohydrolase

3.2.2.6 ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase

3.2.2.7 adenosine nucleosidase

3.2.2.8 ribosylpyrimidine nucleosidase

3.2.2.9 adenosylhomocysteine nucleosidase

3.2.2.10 pyrimidine-5′-nucleotide nucleosidase

3.2.2.11 β-aspartyl-N-acetylglucosaminidase

3.2.2.12 inosinate nucleosidase

3.2.2.13 1-methyladenosine nucleosidase

3.2.2.14 NMN nucleosidase

3.2.2.15 DNA-deoxyinosine glycosylase

3.2.2.16 methylthioadenosine nucleosidase

3.2.2.17 deoxyribodipyrimidine endonucleosidase

3.2.2.18 created 1984, deleted 1989

3.2.2.19 [protein ADP-ribosylarginine] hydrolase

3.2.2.20 DNA-3-methyladenine glycosylase I

3.2.2.21 DNA-3-methyladenine glycosylase II

3.2.2.22 rRNA N-glycosylase

3.2.2.23 DNA-formamidopyrimidine glycosylase

3.2.2.24 ADP-ribosyl-[dinitrogen reductase] hydrolase

3.2.2.25 N-methyl nucleosidase

3.2.2.26 futalosine hydrolase

3.2.2.27 uracil-DNA glycosylase

3.2.2.28 double-stranded uracil-DNA glycosylase

3.2.2.29 thymine-DNA glycosylase

3.2.2.30 aminodeoxyfutalosine nucleosidase

3.2.2.31 adenine glycosylase

3.2.3 Hydrolysing S-glycosyl compounds (deleted sub-subclass)

3.3 Acting on ether bonds

3.4 Acting on peptide bonds (peptidases)

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.6 Acting on acid anhydrides

3.7 Acting on carbon-carbon bonds

3.8 Acting on halide bonds

3.9 Acting on phosphorus-nitrogen bonds

3.10 Acting on sulfur-nitrogen bonds

3.11 Acting on carbon-phosphorus bonds

3.12 Acting on sulfur-sulfur bonds

3.13 Acting on carbon-sulfur bonds

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Release 2023.1 (January 2023)