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EC 3.5.1.87 Details
show Brenda entryEC number
3.5.1.87
Accepted name
N-carbamoyl-L-amino-acid hydrolase
Reaction
an N-carbamoyl-L-2-amino acid (a 2-ureido carboxylate) + H2O = an L-2-amino acid + NH3 + CO2
Other name(s)
N-carbamyl L-amino acid amidohydrolase,
N-carbamoyl-L-amino acid amidohydrolase,
L-N-carbamoylase,
N-carbamoylase (ambiguous)
Systematic name
N-carbamoyl-L-amino-acid amidohydrolase
Comment
This enzyme, along with EC 3.5.1.77 (N-carbamoyl-D-amino-acid hydrolase), EC 5.1.99.5 (hydantoin racemase) and hydantoinase, forms part of the reaction cascade known as the "hydantoinase process", which allows the total conversion of D,L-5-monosubstituted hydantoins into optically pure D- or L-amino acids [3]. The enzyme from Alcaligenes xylosoxidans has broad specificity for carbamoyl-L-amino acids, although it is inactive on the carbamoyl derivatives of glutamate, aspartate, arginine, tyrosine or tryptophan. The enzyme from Sinorhizobium meliloti requires a divalent cation for activity and can hydrolyse N-carbamoyl-L-tryptophan as well as N-carbamoyl L-amino acids with aliphatic substituents [2]. The enzyme is inactive on derivatives of D-amino acids. In addition to N-carbamoyl L-amino acids, the enzyme can also hydrolyse formyl and acetyl derivatives to varying degrees [1,2].
History
created 2001, modified 2008
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1 Oxidoreductases
3 Hydrolases
3.5.1.34 created 1972, deleted 1981 3.5.1.37 created 1972, deleted 1976 3.5.1.45 created 1978, deleted 1986 3.5.1.80 created 1999, deleted 2002
