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EC 1.5.3.13 Details
show Brenda entryEC number
1.5.3.13
Accepted name
N1-acetylpolyamine oxidase
Reaction
(1) N1-acetylspermidine + O2 + H2O = putrescine + 3-acetamidopropanal + H2O2;; (2) N1-acetylspermine + O2 + H2O = spermidine + 3-acetamidopropanal + H2O2
Other name(s)
hPAO-1,
PAO (ambiguous),
mPAO,
hPAO,
polyamine oxidase (ambiguous)
Systematic name
N1-acetylpolyamine:oxygen oxidoreductase (3-acetamidopropanal-forming)
Comment
The enzyme also catalyses the reaction: N1,N12-diacetylspermine + O2 + H2O = N1-acetylspermidine + 3-acetamamidopropanal + H2O2 [1]. No or very weak activity with spermine, or spermidine in absence of aldehydes. In presence of aldehydes the enzyme catalyses the reactions: 1. spermine + O2 + H2O = spermidine + 3-aminopropanal + H2O2, and with weak efficiency 2. spermidine + O2 + H2O = putrescine + 3-aminopropanal + H2O2 [2]. A flavoprotein (FAD). This enzyme, encoded by the PAOX gene, is found in mammalian peroxisomes and oxidizes N1-acetylated polyamines at the exo (three-carbon) side of the secondary amine, forming 3-acetamamidopropanal. Since the products of the reactions are deacetylated polyamines, this process is known as polyamine back-conversion. Differs in specificity from EC 1.5.3.14 [polyamine oxidase (propane-1,3-diamine-forming)], EC 1.5.3.15 [N8-acetylspermidine oxidase (propane-1,3-diamine-forming)], EC 1.5.3.16 (spermine oxidase) and EC 1.5.3.17 (non-specific polyamine oxidase).
History
created 2009
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1 Oxidoreductases
1.1.1 With NAD+ or NADP+ as acceptor
1.1.99.15 created 1978, deleted 1980 1.1.99.17 created 1982, deleted 2003 1.5.3.3 created 1961, deleted 1972 1.5.3.8 created 1989, deleted 1992 1.5.3.9 created 1989, modified 1999, deleted 2002
