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EC 1.5.3.5 Details
show Brenda entryEC number
1.5.3.5
Accepted name
(S)-6-hydroxynicotine oxidase
Reaction
(S)-6-hydroxynicotine + H2O + O2 = 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + H2O2 (overall reaction);;(1a) (S)-6-hydroxynicotine + O2 = 5-(N-methyl-4,5-dihydro-1H-pyrrol-2-yl)pyridin-2-ol + H2O2;;(1b) 5-(N-methyl-4,5-dihydro-1H-pyrrol-2-yl)pyridin-2-ol + H2O = 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one (spontaneous)
Other name(s)
L-6-hydroxynicotine oxidase,
6-hydroxy-L-nicotine oxidase,
6-hydroxy-L-nicotine:oxygen oxidoreductase,
nctB (gene name)
Systematic name
(S)-6-hydroxynicotine:oxygen oxidoreductase
CAS registry number
37256-29-4
Comment
A flavoprotein (FAD). The enzyme, which participates in nicotine degradation, is specific for the (S) isomer of 6-hydroxynicotine. The bacterium Arthrobacter nicotinovorans, in which this enzyme was originally discovered, has a different enzyme that catalyses a similar reaction with the less common (R)-isomer (cf. EC 1.5.3.6, (R)-6-hydroxynicotine oxidase).
History
created 1972, modified 2015
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1 Oxidoreductases
1.1.1 With NAD+ or NADP+ as acceptor
1.1.99.15 created 1978, deleted 1980 1.1.99.17 created 1982, deleted 2003 1.5.3.3 created 1961, deleted 1972 1.5.3.8 created 1989, deleted 1992 1.5.3.9 created 1989, modified 1999, deleted 2002
