6.4.1.4: methylcrotonoyl-CoA carboxylase

This is an abbreviated version!
For detailed information about methylcrotonoyl-CoA carboxylase, go to the full flat file.

Word Map on EC 6.4.1.4

Reaction

ATP
+
3-methylcrotonoyl-CoA
+
HCO3-
+
H+
=
ADP
+
phosphate
+
3-methylglutaconyl-CoA

Synonyms

Carboxylase, methylcrotonyl coenzyme A, Methylcrotonyl coenzyme A carboxylase, beta-Methylcrotonyl coenzyme A carboxylase, beta-methylcrotonyl CoA carboxylase, Mcase, 3-methylcrotonyl-coenzyme A carboxylase, MCCase, 3-methylcrotonyl-CoA carboxylase, glutaconyl-CoA decarboxylase, glutaconyl-CoA:biotin carboxytransferase, methylcrotonyl-CoA carboxylase, beta-methylcrotonyl-CoA carboxylase, beta-MCC, 3-methylcrotonyl-CoA:carbon-dioxide ligase (ADP-forming), MCC, MCCC, 3-methylcrotonyl coenzyme A carboxylase, MC-CoA carboxylase, 3-methylcrotonyl CoA carboxylase, 3-MCC, MCCA, MCCB, PaMCC, 3-methylcrotonyl-coenzyme A, Pa-MCCase, 3-methylcrotonyl coenzyme-A carboxylase, liuD, liuB, Mcc2

ECTree

     6 Ligases
         6.4 Forming carbon-carbon bonds
             6.4.1 Ligases that form carbon-carbon bonds (only sub-subclass identified to date)
                6.4.1.4 methylcrotonoyl-CoA carboxylase

Cloned

Cloned on EC 6.4.1.4 - methylcrotonoyl-CoA carboxylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA cloned and expressed in Escherichia coli
-
decarboxylase operon cloned and sequenced, overproduced in Escherichia coli
-
expressed in Escherichia coli
expression as fusion proteins in human fibroblasts
-
expression of His-tagged MCCase in Escherichia coli strain BL21
-
MCCase subunits are co-expressed in Escherichia coli in the same plasmid and purified in one step by affinity chromatography using the LiuD-His tag protein interacting with the LiuB-S tag recombinant protein
reverse transcriptase PCR
-
singlecopy gene encoding MCC-A subunit isolated nd characterized
-
the alpha and beta subunits of PaMCC are co-expressed in Escherichia coli as His-tagged fusion proteins
use of baculovirus system to express functionally active human recombinant 3-methylcrotonyl-CoA carboxylase
-