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5.6.1.8: myosin ATPase

This is an abbreviated version!
For detailed information about myosin ATPase, go to the full flat file.

Word Map on EC 5.6.1.8

Reaction

ATP
+
H2O
+
myosin bound to actin filament at position n
=
ADP
+
phosphate
+
myosin bound to actin filament at position n+1

Synonyms

actin activated myosin ATPase, actin-stimulated myosin ATPase, actomyosin, actomyosin 1b ATPase, actomyosin ATPase, actomyosin-ATPase, EC 3.6.1.32, EC 3.6.4.1, heavy meromyosin, heavy meromyosin ATPase, HMM, HMM ATPase, matpase, myofibril ATPase, myosin, myosin 2, myosin 2 motor domain, myosin ATPase, myosin II ATPase, myosin II S1, myosin Mg-ATPase, myosin MgATPase, myosin S1 ATPase, myosin-2, myosin-2 ATPase, myosin-ATPase, natural actomyosin, NMIIB, S1-myosin ATPase

ECTree

     5 Isomerases
         5.6 Isomerases altering macromolecular conformation
             5.6.1 Enzymes altering polypeptide conformation or assembly
                5.6.1.8 myosin ATPase

Purification

Purification on EC 5.6.1.8 - myosin ATPase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, ADP-agarose, hydroxyapatite, BioGel adsorption, myosin Ia and IB
-
immobilized metal ion affinity chromatography (Ni2+)
myofibrils are isolated from heart tissue
myosin with unphosphorylated regulatory light chain (20000 Da) from smooth muscle of gizzard; recombinant His-tagged myosin head: immobilized metal ion affinity chromatography (Ni2+)
-
myosin-enriched actomyosin, BioGel, DEAE, myosin II
-
native myosin and myorod from posterior adductor by ammonium sulfate fractionation, dialysis, and gel filtration
-
native myosin from smooth adductor muscle
-
prepared from heart tissue samples
-