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Literature summary for 5.6.1.8 extracted from
- Myosins from Acanthamoeba castellanii (1982), Methods Enzymol., 85, 357-363.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| actin | - |
Acanthamoeba castellanii |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14000 | - |
alpha,beta,gamma, 1 * 130000 + 1 * 17000 + 1 * 14000, myosin IA, one heavy chain and two light chains, SDS-PAGE | Acanthamoeba castellanii |
| 14000 | - |
alpha,beta,gamma, 1 * 125000 + 1 * 27000 + 1 * 14000, myosin IB, one heavy chain and two light chains, ATP catalytic-, actin binding- and phosphorylation site are located on heavy chain, SDS-PAGE | Acanthamoeba castellanii |
| 17000 | - |
alpha,beta,gamma, 1 * 130000 + 1 * 17000 + 1 * 14000, myosin IA, one heavy chain and two light chains, SDS-PAGE | Acanthamoeba castellanii |
| 17000 | - |
alpha2,beta2,gamma2, 2 * 170000 + 2 * 17500 + 2 * 17000, two heavy chains and four light chains, SDS-PAGE | Acanthamoeba castellanii |
| 17500 | - |
alpha2,beta2,gamma2, 2 * 170000 + 2 * 17500 + 2 * 17000, two heavy chains and four light chains, SDS-PAGE | Acanthamoeba castellanii |
| 27000 | - |
alpha,beta,gamma, 1 * 125000 + 1 * 27000 + 1 * 14000, myosin IB, one heavy chain and two light chains, ATP catalytic-, actin binding- and phosphorylation site are located on heavy chain, SDS-PAGE | Acanthamoeba castellanii |
| 125000 | - |
alpha,beta,gamma, 1 * 125000 + 1 * 27000 + 1 * 14000, myosin IB, one heavy chain and two light chains, ATP catalytic-, actin binding- and phosphorylation site are located on heavy chain, SDS-PAGE | Acanthamoeba castellanii |
| 130000 | - |
alpha,beta,gamma, 1 * 130000 + 1 * 17000 + 1 * 14000, myosin IA, one heavy chain and two light chains, SDS-PAGE | Acanthamoeba castellanii |
| 150000 | - |
myosin IA and IB, sucrose density gradient and eqilibrium centrifugation | Acanthamoeba castellanii |
| 170000 | - |
alpha2,beta2,gamma2, 2 * 170000 + 2 * 17500 + 2 * 17000, two heavy chains and four light chains, SDS-PAGE | Acanthamoeba castellanii |
| 400000 | - |
myosin II, sucrose density gradient and eqilibrium centrifugation | Acanthamoeba castellanii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + myosin bound to actin filament at position n | Acanthamoeba castellanii | - |
ADP + phosphate + myosin bound to actin filament at position n+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acanthamoeba castellanii | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | increasing levels of myosin light chain phosphorylation in cold stressed rats, 1.9 mol of P/mol of myosin, heavy chain of myosin IA and IB can be phosphorylated to the extent of 1 mol of P per mol of heavy chain | Acanthamoeba castellanii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, ADP-agarose, hydroxyapatite, BioGel adsorption, myosin Ia and IB | Acanthamoeba castellanii |
| myosin-enriched actomyosin, BioGel, DEAE, myosin II | Acanthamoeba castellanii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.01 | - |
Mg-ATPase activity, myosin II | Acanthamoeba castellanii |
| 0.2 | - |
K+(EDTA)-ATPase activity, myosin II | Acanthamoeba castellanii |
| 0.8 | - |
Ca-ATPase activity, myosin II | Acanthamoeba castellanii |
| 3 | - |
K+(EDTA)-ATPase activity, myosin IA | Acanthamoeba castellanii |
| 4 | - |
K+(EDTA)-ATPase activity, myosin IB | Acanthamoeba castellanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + myosin bound to actin filament at position n | - |
Acanthamoeba castellanii | ADP + phosphate + myosin bound to actin filament at position n+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | alpha2,beta2,gamma2, 2 * 170000 + 2 * 17500 + 2 * 17000, two heavy chains and four light chains, SDS-PAGE | Acanthamoeba castellanii |
| trimer | alpha,beta,gamma, 1 * 130000 + 1 * 17000 + 1 * 14000, myosin IA, one heavy chain and two light chains, SDS-PAGE | Acanthamoeba castellanii |
| trimer | alpha,beta,gamma, 1 * 125000 + 1 * 27000 + 1 * 14000, myosin IB, one heavy chain and two light chains, ATP catalytic-, actin binding- and phosphorylation site are located on heavy chain, SDS-PAGE | Acanthamoeba castellanii |
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