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4.2.1.8: mannonate dehydratase

This is an abbreviated version!
For detailed information about mannonate dehydratase, go to the full flat file.

Word Map on EC 4.2.1.8

Reaction

D-mannonate
=
2-dehydro-3-deoxy-D-gluconate
+
H2O

Synonyms

Altronate hydrolase, Altronic hydro-lyase, CCNA_00566, D-mannonate dehydratase, D-mannonate hydrolase, D-Mannonate hydrolyase, Dehydratase, mannonate, EcManD, ManD, mannonate dehydratase, Mannonate hydrolyase, Mannonic hydrolase, UxuA

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.8 mannonate dehydratase

Engineering

Engineering on EC 4.2.1.8 - mannonate dehydratase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E185D
kcat decreased compared to wild-type, Km decreased compared to wild-type
E185G
kcat increased compared to wild-type, Km slightly decreased compared to wild-type
E186D
kcat decreased compared to wild-type, Km decreased compared to wild-type
E186G
kcat and Km increased compared to wild-type
E63A/S64A
inactive mutant
H32G
kcat and Km increased compared to wild-type
N103A/D109A
kcat decreased compared to wild-type, Km slightly decreased compared to wild-type
N36G
kcat increased compared to wild-type, Km slightly decreased compared to wild-type
W110F
inactive mutant
H311A
the activity of H311A is only 2.4% that of the native wild type enzyme
Y325F
catalytically inactive