4.2.1.53: oleate hydratase

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For detailed information about oleate hydratase, go to the full flat file.

Hydratase, oleate, OA hydratase, OHase, ohyA, oleate hydratase

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Results	in table
7	Activating Compound
2067	AA Sequence
20	Application
1	CAS Registry Number
29	Cloned(Commentary)
14	Cofactor
2	Crystallization (Commentary)
1	Expression
1	General Stability
12	Inhibitors
11	kcat/KM [mM/s]
17	KM Value [mM]
2	Metals/Ions
21	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
44	Organism
2	PDB ID
9	pH Optimum
4	pH Range
14	Protein Variants
7	Purification (Commentary)
2	Reaction
1	Reaction Type
44	Reference
24	Specific Activity [micromol/min/mg]
1	Storage Stability
141	Substrates and Products (Substrate)
18	Subunits
16	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
3	Temperature Range [°C]
3	Temperature Stability [°C]
12	Turnover Number [1/s]

Purification on EC 4.2.1.53 - oleate hydratase

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by three steps of gel filtration. Recombinant oleate hydratase fused to an N-terminal His tag purified onh Ni-resin

His-Trap column chromatography

His-Trap column chromatography, and gel filtration