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4.2.1.53: oleate hydratase

This is an abbreviated version!
For detailed information about oleate hydratase, go to the full flat file.

Word Map on EC 4.2.1.53

Reaction

(R)-10-hydroxystearate
=
oleate
 +
H2O

Synonyms

Hydratase, oleate, OA hydratase, OHase, ohyA, oleate hydratase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.53 oleate hydratase

Specific Activity

Specific Activity on EC 4.2.1.53 - oleate hydratase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.014
-
with myristoleic acid as substrate, at pH 6.5 and 35°C
0.033
-
with gamma-linolenic acid as substrate, at pH 6.5 and 35°C
0.14
at 30°C, in 20 mM Tris buffer, pH 8, 50 mM NaCl, with mixing speed of 1400 rpm
0.15
alpha-linolenic acid, pH 7.2, 28°C
0.156
linoleic acid, pH 7.2, 28°C
0.16
at 22°C, in 20 mM Tris buffer, pH 8, 50 mM NaCl, with mixing speed of 1400 rpm
0.17
at 30°C, in 20 mM Tris buffer, pH 8, 50 mM NaCl, with mixing speed of 1000 rpm
0.34
at 22°C, in 20 mM Tris buffer, pH 8, 50 mM NaCl, with mixing speed of 2200 rpm, in the presence of 2.5% isopropyl alcohol
0.39
at 22°C, in 20 mM Tris buffer, pH 8, 50 mM NaCl, with mixing speed of 2200 rpm
0.54
-
with alpha-linolenic acid as substrate, at pH 6.5 and 35°C
0.615
gamma-linolenic acid, pH 7.2, 28°C
0.91
-
with linoleic acid as substrate, at pH 6.5 and 35°C
1.205
palmitoleic acid, pH 7.2, 28°C
1.266
oleate, pH 7.2, 28°C
1.95
-
with palmitoleic acid as substrate, at pH 6.5 and 35°C
110
-
with linoleate as substrate, at pH 6.5 and 35°C
1580
-
with oleate as substrate, at pH 6.5 and 35°C
160
-
with gamma-linolenate as substrate, at pH 6.5 and 35°C
20
-
with alpha-linolenate as substrate, at pH 6.5 and 35°C
3.3
at pH 6.5 and 25°C
3.69
-
with oleate as substrate, at pH 6.5 and 35°C
30
-
with myristoleate as substrate, at pH 6.5 and 35°C
550
-
with palmitoleate as substrate, at pH 6.5 and 35°C
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