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4.2.1.53: oleate hydratase

This is an abbreviated version!
For detailed information about oleate hydratase, go to the full flat file.

Word Map on EC 4.2.1.53

Reaction

(R)-10-hydroxystearate
=
oleate
 +
H2O

Synonyms

Hydratase, oleate, OA hydratase, OHase, ohyA, oleate hydratase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.53 oleate hydratase

Engineering

Engineering on EC 4.2.1.53 - oleate hydratase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E50A
the mutant shows reduced activity and FAD binding compared to the wild type enzyme
S34A
the mutant shows reduced activity and FAD binding compared to the wild type enzyme
E50A
-
the mutant shows reduced activity and FAD binding compared to the wild type enzyme
-
S34A
-
the mutant shows reduced activity and FAD binding compared to the wild type enzyme
-
M77E
strong decrease (81%) in the conversion efficiency from oleic acid to 10-hydroxystearic acid
V393T
strong decrease (90%) in the conversion efficiency from oleic acid to 10-hydroxystearic acid
M77E
-
strong decrease (81%) in the conversion efficiency from oleic acid to 10-hydroxystearic acid
-
V393T
-
strong decrease (90%) in the conversion efficiency from oleic acid to 10-hydroxystearic acid
-