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2.5.1.9: riboflavin synthase

This is an abbreviated version!
For detailed information about riboflavin synthase, go to the full flat file.

Word Map on EC 2.5.1.9

Reaction

2 6,7-dimethyl-8-(1-D-ribityl)lumazine =

riboflavin
+
4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine

Synonyms

heavy riboflavin synthase, light riboflavin synthase, lumazine synthase/riboflavin synthase complex, RibD, RibE1, riboflavin synthase, riboflavin synthetase, riboflavine synthase, riboflavine synthetase, synthase, riboflavin

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.9 riboflavin synthase

Storage Stability

Storage Stability on EC 2.5.1.9 - riboflavin synthase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, completely purified enzyme, stable for several months
-
-20°C, slow decomposition by formation of large beta-subunit aggregates devoid of alpha-subunits
-
-90°C or in liquid N2, stable for several months
-
0-4°C, stable for 1 week in presence of saturated ammonium sulfate
-
4°C, 6 months, no loss of activity
-
frozen, mycelium, several months without loss of activity
-
frozen, partially purified in dissolved ammonium sulfate precipitation pellet, stable several weeks
-
solution in 0.1 M phosphate, pH 7.0, 10 mM EDTA and 10 mM sodium sulfite, stable for several months
-