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Literature summary for 2.5.1.9 extracted from

  • Bacher, A.
    Heavy riboflavin synthase from Bacillus subtilis (1986), Methods Enzymol., 122, 192-199.
    View publication on PubMed
Search for more literature for 2.5.1.9

Crystallization (Commentary)

Crystallization (Comment) Organism
large crystals by vapour diffusion method, initial solution: 0.7 M sodium/potassium phosphate, pH 8.7, 0.3 mM 5-nitroso-6-ribitylamino-2,4(1H,3H)-pyrimidinedione, protein 2 mg/ml, reservoir solution: 1.3 M sodium/potassium phosphate, pH 8.7, X-ray structure determination and analysis Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 6,7-dimethyl-8-(1'-D-ribityl)lumazine
-
 Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23500
-
 60 * 16000-16200 (beta) + 3 * 23500 (alpha), heavy riboflavin synthase, X-ray studies, analytical ultracentrifugation Bacillus subtilis
70000
-
 light enzyme Bacillus subtilis
100000
-
 heavy riboflavin synthase Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Bacillus subtilis
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heavy enzyme Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus subtilis

Storage Stability

Storage Stability Organism
-20°C, slow decomposition by formation of large beta-subunit aggregates devoid of alpha-subunits Bacillus subtilis
solution in 0.1 M phosphate, pH 7.0, 10 mM EDTA and 10 mM sodium sulfite, stable for several months Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Bacillus subtilis riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
additional information dismutation of 6,7-dimethyl-8-ribityllumazine yielding riboflavin and 5-amino-6-ribitylamino-2,4-pyrimidinedione is catalyzed by the alpha-subunit Bacillus subtilis ?
-
 ?

Subunits

Subunits Comment Organism
polymer 60 * 16000-16200 (beta) + 3 * 23500 (alpha), heavy riboflavin synthase, X-ray studies, analytical ultracentrifugation Bacillus subtilis
polymer primary structure of beta subunit Bacillus subtilis