Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2,3-dimercapto-1-propanol | enhances activity | Saccharomyces cerevisiae | |
2,3-dimercapto-1-propanol | enhances activity | Eremothecium gossypii | |
2-mercaptoethanol | enhances activity | Saccharomyces cerevisiae | |
2-mercaptoethanol | enhances activity | Eremothecium gossypii | |
N-acetylcysteine | enhances activity | Saccharomyces cerevisiae | |
N-acetylcysteine | enhances activity | Eremothecium gossypii | |
SO32- | enhances activity | Saccharomyces cerevisiae | |
SO32- | enhances activity | Eremothecium gossypii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
6,7-dimethyl-8-(1'-D-ribityl)lumazine | - |
Eremothecium gossypii | |
0.04 | - |
6,7-dimethyl-8-(1'-D-ribityl)lumazine | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine | Escherichia coli | - |
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | - |
? | |
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine | Saccharomyces cerevisiae | - |
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | - |
? | |
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine | Eremothecium gossypii | - |
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | - |
? | |
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine | Eremothecium gossypii AG33 | - |
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eremothecium gossypii | - |
- |
- |
Eremothecium gossypii AG33 | - |
- |
- |
Escherichia coli | - |
strain ATCC 9637 | - |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Saccharomyces cerevisiae |
- |
Eremothecium gossypii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | reaction mechanism | Escherichia coli | |
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | reaction mechanism | Saccharomyces cerevisiae | |
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | reaction mechanism | Eremothecium gossypii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Eremothecium gossypii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
additional information | - |
- |
Eremothecium gossypii |
0.003 | - |
- |
Escherichia coli |
0.052 | - |
purified enzyme | Saccharomyces cerevisiae |
0.266 | - |
purified enzyme | Eremothecium gossypii |
Storage Stability | Organism |
---|---|
-20°C, completely purified enzyme, stable for several months | Saccharomyces cerevisiae |
frozen, partially purified in dissolved ammonium sulfate precipitation pellet, stable several weeks | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine | - |
Escherichia coli | riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | - |
? | |
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine | - |
Saccharomyces cerevisiae | riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | - |
? | |
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine | - |
Eremothecium gossypii | riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | - |
? | |
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine | - |
Eremothecium gossypii AG33 | riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | - |
? | |
additional information | - |
Escherichia coli | ? | - |
? | |
additional information | overview | Saccharomyces cerevisiae | ? | - |
? | |
additional information | overview | Eremothecium gossypii | ? | - |
? | |
additional information | overview | Eremothecium gossypii AG33 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
37 | - |
assay at | Saccharomyces cerevisiae |
37 | - |
assay at | Eremothecium gossypii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
2 | cold lability, inclusion of 0.01 M sodium sulfite in the solution used in purification prevents inactivation | Saccharomyces cerevisiae |
92 | - |
1 min, complete loss of activity | Eremothecium gossypii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
- |
Eremothecium gossypii |
7 | - |
- |
Escherichia coli |
7 | - |
- |
Saccharomyces cerevisiae |