1.5.1.36: flavin reductase (NADH)
This is an abbreviated version!
For detailed information about flavin reductase (NADH), go to the full flat file.
Word Map on EC 1.5.1.36
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1.5.1.36
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nadph:flavin
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fmnh2
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alkanesulfonate
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oxygen-insensitive
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beneckea
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desulfonation
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nitroreductases
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fmnh2-dependent
- 1.5.1.36
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nadph:flavin
- fmnh2
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alkanesulfonate
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oxygen-insensitive
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beneckea
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desulfonation
- nitroreductases
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fmnh2-dependent
Reaction
Synonyms
AbeF, BaiH, BorF, C1-HpaH, DszD, FAD reductase, FerA, flavin mononucleotide reductase, flavin reductase, flavin:NADH oxidoreductase, FMN reductase, Frd188, frd2, fre, HpaC, LJ0548, LJ0549, LJ_0548, LJ_0549, LuxG, More, NAD(P)H-dependent H2O2-forming flavin reductase, NAD(P)H-flavin oxidoreductase, NAD(P)H:flavin oxidoreductase, NAD(P)H:flavin-oxidoreductase, NADH-dependent flavin reductase, NADH-flavin oxidoreductase, NADH: flavin oxidoreductase, NADH: flavinoxidore ductase/NADH oxidase, NADH:flavin oxidoreductase, NADH:FMN oxidoreductase, nfr1, nfr2, NOX, Pden2689, SMOB-ADP1
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Purification
Purification on EC 1.5.1.36 - flavin reductase (NADH)
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ammonium sulfate precipitation, butyl-Sepharose column chromatography, Source 30Q column chromatography, FMN-Sepharose 6B affinity column chromatography, and Superdex 75 gel filtration
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ammonium sulfate precipitation, HiTrap Q column chromatography, and Superdex 200 gel filtration
native enzyme from strain NCC 533 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography
recombinant His-tagged wild-type enzymes HpaC and Fre from strain BL21(DE3)
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recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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refolded recombinant enzyme by anion exchange chromatography and gel filtration, or by nickel affinity chromatography and gel filtration, when a His10-tagged enzyme is expressed