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Literature summary for 1.5.1.36 extracted from
- Expression of the bile acid-inducible NADH flavin oxidoreductase gene of Eubacterium sp. VPI 12708 in Escherichia coli (1995), Biochim. Biophys. Acta, 1249, 145-154 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| subcloned into an IPTG-inducible expression vector, pBaiH2.2. Escherichia coli DH5a cells transformed with pBaiH2.2 express 10fold higher levels of NADH:FOR upon induction with IPTG than did Eubacterium sp. VPI 12708 cells induced with cholic acid | [Clostridium] scindens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
[Clostridium] scindens |  |
| Acriflavine | - |
[Clostridium] scindens | |
| CuCl2 | - |
[Clostridium] scindens | |
| EDTA | - |
[Clostridium] scindens | |
| EGTA | - |
[Clostridium] scindens | |
| HgCl2 | - |
[Clostridium] scindens | |
| additional information | iodoacetate and iodoacetamide show no inhibition | [Clostridium] scindens | |
| N-bromosuccinimide | - |
[Clostridium] scindens | |
| N-ethylmaleimide | - |
[Clostridium] scindens | |
| p-chloromercuriphenylsulfonic acid | - |
[Clostridium] scindens | |
| Sodium azide | - |
[Clostridium] scindens | |
| ZnCl2 | - |
[Clostridium] scindens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| copper | contains 1 mol of copper per subunit | [Clostridium] scindens | |
| Iron | contains up to 2 mol of iron per subunit | [Clostridium] scindens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 210000 | - |
non-denaturing PAGE | [Clostridium] scindens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Clostridium] scindens | P32370 | - |
- |
| [Clostridium] scindens VPI 12708 | P32370 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
[Clostridium] scindens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| reduced flavin + NAD+ | - |
[Clostridium] scindens | flavin + NADH + H+ | - |
? | |
| reduced flavin + NAD+ | - |
[Clostridium] scindens JCM 10418 | flavin + NADH + H+ | - |
? | |
| reduced flavin + NAD+ | - |
[Clostridium] scindens VPI 12708 | flavin + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | 3 * 71400 | [Clostridium] scindens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BaiH | - |
[Clostridium] scindens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
[Clostridium] scindens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
[Clostridium] scindens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains 1 mol of FAD | [Clostridium] scindens | |
| NADH | - |
[Clostridium] scindens | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| [Clostridium] scindens | isoelectric focusing | - |
6 |
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