1.2.1.107: glyceraldehyde-3-phosphate dehydrogenase (arsenate-transferring)
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For detailed information about glyceraldehyde-3-phosphate dehydrogenase (arsenate-transferring), go to the full flat file.
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Synonyms
acylglyceraldehyde-3-phosphate dehydrogenase, AsV reductase, D-glyceraldehyde-3-phosphate dehydrogenase, FA-GPDH, G3P dehydrogenase, G3PD, G3PDH, GAPDH, glyceraldehyde-3-P dehydrogenase, GPD, [beta-(2-furyl)acryloyl]glyceraldehyde-3-phosphate dehydrogenase
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Engineering
Engineering on EC 1.2.1.107 - glyceraldehyde-3-phosphate dehydrogenase (arsenate-transferring)
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H176N
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the mutant shows a Km value 5times smaller than for the wild type enzyme while the Km value for arsenate is about 5times larger. The mutant has a significantly reduced thermal stability at 65°C. The half-life for thermal denaturation for the mutant holoenzyme is increased nearly 100fold over the corresponding value for the apoenzyme