1.14.16.5: alkylglycerol monooxygenase

This is an abbreviated version!
For detailed information about alkylglycerol monooxygenase, go to the full flat file.

Word Map on EC 1.14.16.5

Reaction

1-O-alkyl-sn-glycerol
+
tetrahydrobiopterin
+
O2
=
1-O-(1-hydroxyalkyl)-sn-glycerol
+
dihydrobiopterin
+
H2O

Synonyms

AGMO, alkylglycerol mono-oxygenase, alkylglycerol monooxygenase, EC 1.14.99.17, glyceryl ether hydroxylase, glyceryl ether monooxygenase, glyceryl ether oxidase, glyceryl ether oxygenase, glyceryl ether-cleaving enzyme, glyceryl etherase, glyceryl-ether cleaving enzyme, O-alkylglycerol monooxygenase, oxygenase, glyceryl ether mono-, PRUB680

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.16.5 alkylglycerol monooxygenase

Purification

Purification on EC 1.14.16.5 - alkylglycerol monooxygenase

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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
6-aminohexyl Sepharose column chromatography, diethylaminoethyl cellulose column chromatography, sucrose density centrifugation, and hexadecylglycerol affinity chromatography
-
microsomes from rat liver are prepared
-
partial, 100fold purification of the native enzyme from liver microsomes
-