1.14.14.88: isoflavone 3'-hydroxylase

This is an abbreviated version!
For detailed information about isoflavone 3'-hydroxylase, go to the full flat file.

EC 1.14.13.52, I3'H, isoflavone 3'-hydroxylase, isoflavone 3'-monooxygenase, MtCYP81E9, Mtl3'H

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Results	in table
2	Activating Compound
5	AA Sequence
1	Application
1	CAS Registry Number
2	Cloned(Commentary)
1	Cofactor
1	Expression
1	General Information
8	Inhibitors
4	KM Value [mM]
2	Localization
4	Natural Substrates/ Products (Substrates)
6	Organism
5	Pathway
3	pH Optimum
1	pH Range
1	Reaction
4	Reaction Type
6	Reference
9	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
17	Substrates and Products (Substrate)
7	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
2	Turnover Number [1/s]

Substrates Products on EC 1.14.14.88 - isoflavone 3'-hydroxylase

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2'-hydroxyformononetin + [reduced NADPH-hemoprotein reductase] + O2

2',3'-dihydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O

39% of activity with biochanin A

biochanin A + [reduced NADPH-hemoprotein reductase] + O2

biochanin A + [reduced NADPH-hemoprotein reductase] + O2

pratensein + [oxidized NADPH-hemoprotein reductase] + H2O

formononetin + [reduced NADPH-hemoprotein reductase] + O2

3'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O

formononetin + [reduced NADPH-hemoprotein reductase] + O2

calycosin + [oxidized NADPH-hemoprotein reductase] + H2O

genistein + NADPH + O2

19% of activity with biochanin A

additional information