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Literature summary for 1.14.14.88 extracted from
- Regiospecific hydroxylation of isoflavones by cytochrome p450 81E enzymes from Medicago truncatula (2003), Plant J., 36, 471-484.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
formononetin | pH 8.0, 2 mM NADPH | Medicago truncatula |  |
| 0.11 | - |
Biochanin A | pH 8.0, 2 mM NADPH | Medicago truncatula | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum membrane | - |
Medicago truncatula | 5789 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Medicago truncatula | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | low mRNA levels | Medicago truncatula | - |
| root | MRNA is found after arbuscular mycorrhizal colonization | Medicago truncatula | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-hydroxyformononetin + [reduced NADPH-hemoprotein reductase] + O2 | 39% of activity with biochanin A | Medicago truncatula | 2',3'-dihydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| biochanin A + [reduced NADPH-hemoprotein reductase] + O2 | - |
Medicago truncatula | ? | - |
? | |
| formononetin + [reduced NADPH-hemoprotein reductase] + O2 | 50% of activity with biochanin A | Medicago truncatula | calycosin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| genistein + NADPH + O2 | 19% of activity with biochanin A | Medicago truncatula | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MtCYP81E9 | - |
Medicago truncatula |
| Mtl3'H | - |
Medicago truncatula |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.028 | - |
formononetin | pH 8.0, 2 mM NADPH | Medicago truncatula | |
| 0.1 | - |
Biochanin A | pH 8.0, 2 mM NADPH | Medicago truncatula | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Medicago truncatula |
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Release 2023.1 (January 2023)
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