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1.14.13.127: 3-(3-hydroxyphenyl)propanoate hydroxylase

This is an abbreviated version!
For detailed information about 3-(3-hydroxyphenyl)propanoate hydroxylase, go to the full flat file.

Reaction

(2E)-3-(3-hydroxyphenyl)prop-2-enoate
+
NADH
+
H+
+
O2
=
(2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate
+
H2O
+
NAD+

Synonyms

3-(3-hydroxyphenyl)propionate hydroxylase, 3HPP 2-hydroxylase, 3HPP hydroxylase, MhpA, MhpAK-12

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.127 3-(3-hydroxyphenyl)propanoate hydroxylase

Purification

Purification on EC 1.14.13.127 - 3-(3-hydroxyphenyl)propanoate hydroxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant C-terminally His-tagged enzyme from Escherichia coli strain Rosetta(DE3)pLysS(pET-28a-mhpA) by nickel affinity chromatography