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Literature summary for 1.14.13.127 extracted from
- MhpA is a hydroxylase catalyzing the initial reaction of 3-(3-hydroxyphenyl)propionate catabolism in Escherichia coli K-12 (2020), Appl. Environ. Microbiol., 86, e02385-19 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene mhpA, genetic organization and comparison of the genetic organization of 3-(3-hydroxyphenyl)propanoate (3HPP) catabolic clusters, sequence comparisons and phylogenetic analysis and tree, enzyme expression analysis, recombinant overexpression of C-terminally His-tagged enzyme in Escherichia coli strain Rosetta(DE3)pLysS(pET-28a-mhpA) | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of truncated versions MhpAK-12_400 and MhpAK-12_480 (with 154 and 74 residues deleted from the C-terminus, respectively), the mutants both lose their activities | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.381 | - |
NADH | pH 7.4, 22°C, recombinant wild-type enzyme | Escherichia coli |  |
| 0.437 | - |
NADPH | pH 7.4, 22°C, recombinant wild-type enzyme | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 669000 | - |
recombinant C-terminally His-tagged enzyme, gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-(3-hydroxyphenyl)propanoate + NADH + H+ + O2 | Escherichia coli | - |
3-(2,3-dihydroxyphenyl)propanoate + H2O + NAD+ | - |
? | |
| 3-(3-hydroxyphenyl)propanoate + NADH + H+ + O2 | Escherichia coli W3110 | - |
3-(2,3-dihydroxyphenyl)propanoate + H2O + NAD+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P77397 | - |
- |
| Escherichia coli W3110 | P77397 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His-tagged enzyme from Escherichia coli strain Rosetta(DE3)pLysS(pET-28a-mhpA) by nickel affinity chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.61 | - |
purified recombinant enzyme, with cofactor NADPH, pH 7.4, 22°C | Escherichia coli |
| 0.94 | - |
purified recombinant enzyme, with cofactor NADH, pH 7.4, 22°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-(3-hydroxyphenyl)propanoate + NADH + H+ + O2 | - |
Escherichia coli | 3-(2,3-dihydroxyphenyl)propanoate + H2O + NAD+ | - |
? | |
| 3-(3-hydroxyphenyl)propanoate + NADH + H+ + O2 | - |
Escherichia coli W3110 | 3-(2,3-dihydroxyphenyl)propanoate + H2O + NAD+ | - |
? | |
| 3-(3-hydroxyphenyl)propanoate + NADPH + H+ + O2 | - |
Escherichia coli | 3-(2,3-dihydroxyphenyl)propanoate + H2O + NADP+ | - |
? | |
| 3-(3-hydroxyphenyl)propanoate + NADPH + H+ + O2 | - |
Escherichia coli W3110 | 3-(2,3-dihydroxyphenyl)propanoate + H2O + NADP+ | - |
? | |
| additional information | substrate and product identification by mass spectrometry | Escherichia coli | ? | - |
- |
|
| additional information | substrate and product identification by mass spectrometry | Escherichia coli W3110 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| multimer | x * 61000, recombinant C-terminally His-tagged enzyme, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3HPP 2-hydroxylase | - |
Escherichia coli |
| MhpA | - |
Escherichia coli |
| MhpAK-12 | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.691 | - |
NADH | pH 7.4, 22°C, recombinant wild-type enzyme | Escherichia coli | |
| 0.736 | - |
NADPH | pH 7.4, 22°C, recombinant wild-type enzyme | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | tightly bound to the enzyme, a FAD-binding domain is located in the N-terminus | Escherichia coli | |
| additional information | using NADH or NADPH as a cofactor, purified MhpAK-12 catalyzes the conversion of 3HPP to DHPP at a similar efficiency | Escherichia coli | |
| NADH | - |
Escherichia coli | |
| NADPH | - |
Escherichia coli | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | MhpAK-12 and its putative homologues belong to taxa that are phylogenetically distant from functionally identified FAD-containing monooxygenases (hydroxylases) | Escherichia coli |
| malfunction | gene deletion and complementation showed that mhpA is vital for its growth on 3-(3-hydroxyphenyl)propanoate (3HPP), but the mhpA deletion strain is still able to grow on 3-(2,3-dihydroxyphenyl)propionate (DHPP), the hydroxylation product is transformed from 3HPP by MhpAK-12. MhpAK-12 has approximately an extra 150 residues at its C-terminus in comparison to its close homologues, but its truncated versions MhpAK-12_400 and MhpAK-12_480 (with 154 and 74 residues deleted from the C-terminus, respectively) both lose their activities | Escherichia coli |
| metabolism | proposed pathway for 3-(3-hydroxyphenyl)propanoate (3HPP) degradation by Escherichia coli strain K-12 via the meta pathway, together with the catabolic reactions catalyzed by the mhp gene products in vivo, overview | Escherichia coli |
| additional information | the 154 amino acid residues in the C-terminus of MhpAK-12 play an important role in its hydroxylation activity of the enzyme | Escherichia coli |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.684 | - |
NADPH | pH 7.4, 22°C, recombinant wild-type enzyme | Escherichia coli | |
| 1.813 | - |
NADH | pH 7.4, 22°C, recombinant wild-type enzyme | Escherichia coli | |
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