1.13.12.9: phenylalanine 2-monooxygenase
This is an abbreviated version!
For detailed information about phenylalanine 2-monooxygenase, go to the full flat file.
Word Map on EC 1.13.12.9
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1.13.12.9
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fad
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l-methionine
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proenzyme
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l-tyrosine
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purple
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ammonia
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flavin
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l-amino
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noncatalytic
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l-met
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prosequence
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charge-transfer
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analysis
- 1.13.12.9
- fad
- l-methionine
- proenzyme
- l-tyrosine
-
purple
- ammonia
- flavin
-
l-amino
-
noncatalytic
- l-met
-
prosequence
-
charge-transfer
- analysis
Reaction
Synonyms
L-Phe oxidase, L-phenylalanine oxidase, L-phenylalanine oxidase (deaminating and decarboxylating), oxidase, phenylalanine (deaminating, decarboxylating), PAOpt, proPAO
ECTree
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Purification
Purification on EC 1.13.12.9 - phenylalanine 2-monooxygenase
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cell disruption by sonication, centrifugation, supernatant applied to HisTrap HP column equilibrated with 20 mM Tris-HCl (pH 8.0) containing 500 mM NaCl, elution with linear gradient of imidazole from 0-0.5 M, ammonium sulfate added to eluted fractions, centrifugation of precipitate, solution in 20 mM Tris-HCl (pH 8.0) containing 300 mM ammonium sulfate, several chromatographic steps using HiTrap Phenyl FF, Resource Q, and HiLoad 16/60 Superdex 200 prep grade, activated enzyme purified by HiTrap Q HP and gel filtration
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