1.13.12.9: phenylalanine 2-monooxygenase

This is an abbreviated version!
For detailed information about phenylalanine 2-monooxygenase, go to the full flat file.

Word Map on EC 1.13.12.9

Reaction

L-phenylalanine
+
O2
=
2-phenylacetamide
+
CO2
+
H2O

Synonyms

CC-LPOX, L-Phe oxidase, L-phenylalanine oxidase, L-phenylalanine oxidase (deaminating and decarboxylating), oxidase, phenylalanine (deaminating, decarboxylating), PAOpt, proPAO

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.9 phenylalanine 2-monooxygenase

Purification

Purification on EC 1.13.12.9 - phenylalanine 2-monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
cell disruption by sonication, centrifugation, supernatant applied to HisTrap HP column equilibrated with 20 mM Tris-HCl (pH 8.0) containing 500 mM NaCl, elution with linear gradient of imidazole from 0-0.5 M, ammonium sulfate added to eluted fractions, centrifugation of precipitate, solution in 20 mM Tris-HCl (pH 8.0) containing 300 mM ammonium sulfate, several chromatographic steps using HiTrap Phenyl FF, Resource Q, and HiLoad 16/60 Superdex 200 prep grade, activated enzyme purified by HiTrap Q HP and gel filtration
-
recombinant enzyme using His-tag
-
recombinant His-tagged enzyme 35fold from Escherichia coli strain Rosetta (DE3) by nickel affinity and hydrophbic interaction chromatography