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1.13.11.67: 8'-apo-beta-carotenoid 14',13'-cleaving dioxygenase

This is an abbreviated version!
For detailed information about 8'-apo-beta-carotenoid 14',13'-cleaving dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.67

Reaction

8'-apo-beta-carotenol
+
O2
=
14'-apo-beta-carotenal
 +
7-hydroxy-6-methylhepta-3,5-dienal

Synonyms

carotenoid cleavage dioxygenase, CarT, CCD, EC 1.13.12.12, torulene oxygenase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.67 8'-apo-beta-carotenoid 14',13'-cleaving dioxygenase

Crystallization

Crystallization on EC 1.13.11.67 - 8'-apo-beta-carotenoid 14',13'-cleaving dioxygenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
iron-bound enzyme in complex with 3-hydroxy-beta-apo-12'-carotenal, sitting drop vapor diffusion method, using 0.1 M MES-NaHOH, pH 6.0, 0.1 M NaCl. 22% (v/v) Sokalan PA 25 CL and 22% (w/v) xylitol. Cobalt-bound enzyme in complex with 3-hydroxy-beta-apo-12'-carotenal, sitting drop vapor diffusion method, using 0.1 M MES-NaOH, pH 6.0, 0.1 M NaCl, 22% (v/v) Sokalan PA 25 CL, 5% (v/v) glycerol and 10-13% (w/v) xylitol