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IUBMB Comments A thiol-dependent enzyme isolated from rat and rabbit. Unlike EC 1.13.11.63 , β-carotene-15,15′-dioxygenase, it is not active towards β-carotene. The secondary product has not been characterized, but may be (3E ,5E )-7-hydroxy-6-methylhepta-3,5-dien-2-one.
The expected taxonomic range for this enzyme is: Archaea, Eukaryota
Synonyms carotenoid cleavage dioxygenase, more
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carotenoid cleavage dioxygenase
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EC 1.13.12.12
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formerly
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8'-apo-beta-carotenol + O2 = 14'-apo-beta-carotenal + 7-hydroxy-6-methylhepta-3,5-dienal
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8'-apo-beta-carotenol:O2 oxidoreductase (14',13'-cleaving)
A thiol-dependent enzyme isolated from rat and rabbit. Unlike EC 1.13.11.63, beta-carotene-15,15'-dioxygenase, it is not active towards beta-carotene. The secondary product has not been characterized, but may be (3E,5E)-7-hydroxy-6-methylhepta-3,5-dien-2-one.
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3-hydroxy-beta-apo-12'-carotenal + O2
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Substrates: the enzyme cleaves specifically at the C14'-C13' position Products: -
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8'-apo-beta-carotenol + O2
14'-apo-beta-carotenal + ?
all-trans-8'-apo-beta-carotenal + O2
beta-apo-14'-carotenal + ?
Substrates: the enzyme cleaves specifically at the C14'-C13' position Products: -
?
all-trans-beta-apo-8'-carotenol
14'-apo-carotenal + ?
Substrates: - Products: -
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beta-apo-10'-carotenal
14'-apo-carotenal + ?
Substrates: - Products: -
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beta-apo-10'-carotenal + O2
retinal + ?
Substrates: - Products: -
?
beta-apo-8'-carotenal + O2
(2E,4E,6E,8E,10E)-5,9-dimethyl-11-(2,6,6-trimethylcyclohex-1-en-1-yl)undeca-2,4,6,8,10-pentaenal + (2E,4E)-2-methylhepta-2,4-dienedial
Substrates: - Products: -
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beta-apo-8'-lycopenal + O2
crocetindial + ?
Substrates: - Products: main product
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lycopene + O2
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Substrates: - Products: eccentric cleavage close to the end of lycopene
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torulene + O2
beta-apo-4'-carotenal + 3-methylbut-2-enal
Substrates: - Products: -
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additional information
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8'-apo-beta-carotenol + O2
14'-apo-beta-carotenal + ?
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Substrates: - Products: -
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8'-apo-beta-carotenol + O2
14'-apo-beta-carotenal + ?
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Substrates: - Products: -
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additional information
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Substrates: no activity with beta-carotene, zeaxanthin, lycopene, retinal, resveratrol and isoeugenol Products: -
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additional information
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Substrates: enzyme cleaves at the third double bond from the uncycled tail of carotenoids with preference for non-oxygenated tails Products: -
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Fe2+
the enzyme contains an iron center
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1,10-phenanthroline
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complete inhibition at 1 mM
1,10-phenanthroline
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complete inhibition at 1 mM
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detergent
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detergent is necessary for solubilizing substrates, maximal activity with 0.5% CHAPS or 0.5% sodium cholate
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detergent
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detergent is necessary for solubilizing substrates, maximal activity with 0.5% CHAPS or 0.5% sodium cholate
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glutathione
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thiol-dependent enzyme, decrease of glutathione from 2 to 1 mM decreases activity by 50%
glutathione
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thiol-dependent enzyme, decrease of glutathione from 2 to 1 mM decreases activity by 50%
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Dehydration
Dehydration-Induced Carotenoid Cleavage Dioxygenase 1 Reveals a Novel Route for ?-Ionone Formation during Tea (Camellia sinensis) Withering.
Dehydration
Overexpression of Crocus carotenoid cleavage dioxygenase, CsCCD4b, in Arabidopsis imparts tolerance to dehydration, salt and oxidative stresses by modulating ROS machinery.
Starvation
Genetic Diversity and Genome-Wide Association Study of Major Ear Quantitative Traits Using High-Density SNPs in Maize.
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2 - 11
8'-apo-beta-carotenol
0.0982
all-trans-8'-apo-beta-carotenal
at pH 7.0 and 22°C
0.0182
all-trans-beta-apo-8'-carotenol
at pH 7.0 and 22°C
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2
8'-apo-beta-carotenol
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11
8'-apo-beta-carotenol
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0.11
all-trans-8'-apo-beta-carotenal
at pH 7.0 and 22°C
0.0025
all-trans-beta-apo-8'-carotenol
at pH 7.0 and 22°C
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0.000002
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from intestinal mucosa preparation
0.00002
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from intestinal mucosa preparation
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8
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UniProt
brenda
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UniProt
brenda
rabbit
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brenda
rat
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brenda
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brenda
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brenda
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brenda
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brenda
Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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A0A128A3G4_9ARCH
472
0
53817
TrEMBL
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A1KQY3_GIBFU
577
0
64487
TrEMBL
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?
x * 53817, calculated from amino acid sequence
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iron-bound enzyme in complex with 3-hydroxy-beta-apo-12'-carotenal, sitting drop vapor diffusion method, using 0.1 M MES-NaHOH, pH 6.0, 0.1 M NaCl. 22% (v/v) Sokalan PA 25 CL and 22% (w/v) xylitol. Cobalt-bound enzyme in complex with 3-hydroxy-beta-apo-12'-carotenal, sitting drop vapor diffusion method, using 0.1 M MES-NaOH, pH 6.0, 0.1 M NaCl, 22% (v/v) Sokalan PA 25 CL, 5% (v/v) glycerol and 10-13% (w/v) xylitol
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G71D
mutant accumulates torulene instead of the final product of the pathway, neurosporaxanthin
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52
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30% of control activity after heating to 52°C for 3 min
52
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30% of control activity after heating to 52°C for 3 min
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HiTrap Q column chromatography, MonoQ column chromatography, and Superdex 200 gel filtration
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expressed in Escherichia coli T7 Express cells
expression in Escherichia coli
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Dmitrovskii, A.A.; Gessler, N.N.; Gomboeva, S.B.; Ershov, Y.V.; Bykhovsky, V.Y.
Enzymatic oxidation of beta-apo-8'-carotenol to beta-apo-14'-carotenal by an enzyme different from beta-carotene-15-15'-dioxygenase
Biochemistry (Moscow)
62
787-792
1997
Oryctolagus cuniculus, Rattus norvegicus
brenda
Prado-Cabrero, A.; Estrada, A.F.; Al-Babill, S.; Avalos, J.
Identification and biochemical characterization of a novel carotenoid oxygenase: elucidation of the cleavage step in the Fusarium carotenoid pathway
Mol. Microbiol.
64
448-460
2007
Fusarium fujikuroi (A1KQY3)
brenda
Daruwalla, A.; Zhang, J.; Lee, H.J.; Khadka, N.; Farquhar, E.R.; Shi, W.; von Lintig, J.; Kiser, P.D.
Structural basis for carotenoid cleavage by an archaeal carotenoid dioxygenase
Proc. Natl. Acad. Sci. USA
117
19914-19925
2020
Candidatus Nitrosotalea devanaterra (A0A128A3G4)
brenda
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