Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli T7 Express cells | Candidatus Nitrosotalea devanaterra |
Crystallization (Comment) | Organism |
---|---|
iron-bound enzyme in complex with 3-hydroxy-beta-apo-12'-carotenal, sitting drop vapor diffusion method, using 0.1 M MES-NaHOH, pH 6.0, 0.1 M NaCl. 22% (v/v) Sokalan PA 25 CL and 22% (w/v) xylitol. Cobalt-bound enzyme in complex with 3-hydroxy-beta-apo-12'-carotenal, sitting drop vapor diffusion method, using 0.1 M MES-NaOH, pH 6.0, 0.1 M NaCl, 22% (v/v) Sokalan PA 25 CL, 5% (v/v) glycerol and 10-13% (w/v) xylitol | Candidatus Nitrosotalea devanaterra |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0182 | - |
all-trans-beta-apo-8'-carotenol | at pH 7.0 and 22°C | Candidatus Nitrosotalea devanaterra | |
0.0982 | - |
all-trans-8'-apo-beta-carotenal | at pH 7.0 and 22°C | Candidatus Nitrosotalea devanaterra |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains an iron center | Candidatus Nitrosotalea devanaterra |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candidatus Nitrosotalea devanaterra | A0A128A3G4 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Q column chromatography, MonoQ column chromatography, and Superdex 200 gel filtration | Candidatus Nitrosotalea devanaterra |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-beta-apo-12'-carotenal + O2 | the enzyme cleaves specifically at the C14'-C13' position | Candidatus Nitrosotalea devanaterra | ? | - |
? | |
all-trans-8'-apo-beta-carotenal + O2 | the enzyme cleaves specifically at the C14'-C13' position | Candidatus Nitrosotalea devanaterra | beta-apo-14'-carotenal + ? | - |
? | |
all-trans-beta-apo-8'-carotenol | - |
Candidatus Nitrosotalea devanaterra | 14'-apo-carotenal + ? | - |
? | |
beta-apo-10'-carotenal | - |
Candidatus Nitrosotalea devanaterra | 14'-apo-carotenal + ? | - |
? | |
additional information | no activity with beta-carotene, zeaxanthin, lycopene, retinal, resveratrol and isoeugenol | Candidatus Nitrosotalea devanaterra | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 53817, calculated from amino acid sequence | Candidatus Nitrosotalea devanaterra |
Synonyms | Comment | Organism |
---|---|---|
carotenoid cleavage dioxygenase | - |
Candidatus Nitrosotalea devanaterra |
CCD | - |
Candidatus Nitrosotalea devanaterra |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
all-trans-beta-apo-8'-carotenol | at pH 7.0 and 22°C | Candidatus Nitrosotalea devanaterra | |
0.11 | - |
all-trans-8'-apo-beta-carotenal | at pH 7.0 and 22°C | Candidatus Nitrosotalea devanaterra |