EC Number   |
General Stability |
Reference |
|---|
    2.7.1.40 | 0.1 M KCl stabilizes enzyme in dilute solutions |
641586 |
| 2.7.1.40 | 0.5 M sucrose and 0.1 M KCl stabilize |
641566 |
| 2.7.1.40 | 25 mM MES and 25 mM Bis-Tris propane as the assay buffer |
676689 |
| 2.7.1.40 | at either pH value, the enzyme displays equivalent activity in 50 mM HEPES or a mixture of |
676689 |
| 2.7.1.40 | binding of frucctose 2,6-bisphophate plays the most important role in stabilizing the LmPYK tetramer in solution |
722668 |
| 2.7.1.40 | cytosolic isozyme is much more stable than plastid isozyme, dithiothreitol plus glycerol stabilize plastid isozyme PKp during purification, isozyme PKp requires dithiothreitol or 2-mercaptoethanol during purification, cytosolic isozyme PKc is stable to dilution into 50 mM N-tris[hydroxymethyl]methyl-2-aminoethane-sulfonic acid buffer, pH 7.9 |
641567 |
| 2.7.1.40 | dialysis against 10 mM sodium phosphate or Tris-HCl, pH 7.5, for 18 h at 4°C, inactivates |
641582 |
| 2.7.1.40 | dialysis for 20 h against 40 mM Tris-HCl, Tris-maleate or morpholinepropanesulfonic acid-NaOH buffer, pH 7, inactivates, but stable to dialysis against phosphate buffer, pH 7 |
641589 |
| 2.7.1.40 | even in the presence of glycerol or ethylene glycol, Mg2+ and dithioerythritol, at protein concentrations below 0.4 mg/ml, the enzyme is very unstable during extraction and purification |
641568 |
| 2.7.1.40 | freezing inactivates |
641590 |
