Activating Compound | Comment | Organism | Structure |
---|---|---|---|
aspartate | allosteric activator | Ricinus communis |
General Stability | Organism |
---|---|
25 mM MES and 25 mM Bis-Tris propane as the assay buffer | Ricinus communis |
at either pH value, the enzyme displays equivalent activity in 50 mM HEPES or a mixture of | Ricinus communis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | 80% activity at pH 6.4, 86% activity at pH 7.4 | Ricinus communis | |
ATP | IC50: 8.8 mM at pH 6.4, IC50: 13.7 mM at pH 7.4 | Ricinus communis | |
citrate | IC50: 9.2 mM at pH 6.4, IC50: 14.2 mM at pH 7.4 | Ricinus communis | |
D-fructose 1,6-bisphosphate | IC50: 8.4 mM at pH 6.4, IC50: 8.0 mM at pH 7.4 | Ricinus communis | |
diphosphate | IC50: 9.8 mM at pH 6.4, IC50: 17.2 mM at pH 7.4 | Ricinus communis | |
glutamate | IC50: 2.5 mM at pH 6.4, IC50: 1.2 mM at pH 7.4 | Ricinus communis | |
isocitrate | 76% activity at pH 6.4, 91% activity at pH 7.4 | Ricinus communis | |
malate | 78% activity at pH 6.4, 90% activity at pH 7.4 | Ricinus communis | |
additional information | not inhibited by 5 mM ADP | Ricinus communis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.046 | - |
ADP | at pH 7.4 | Ricinus communis | |
0.046 | - |
phosphoenolpyruvate | at pH 7.4 | Ricinus communis | |
0.055 | - |
ADP | at pH 7.4, in the presence of 10 mM glutamate | Ricinus communis | |
0.082 | - |
phosphoenolpyruvate | at pH 6.4 | Ricinus communis | |
0.09 | - |
ADP | at pH 6.4 | Ricinus communis | |
0.093 | - |
phosphoenolpyruvate | at pH 7.4, in the presence of 10 mM glutamate | Ricinus communis | |
0.101 | - |
ADP | at pH 6.4, in the presence of 10 mM glutamate | Ricinus communis | |
0.15 | - |
phosphoenolpyruvate | at pH 6.4, in the presence of 10 mM glutamate | Ricinus communis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Glycine max | 5829 | - |
cytosol | - |
Ricinus communis | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is independent of free Mg2+ concentration in the range 4-20 mM | Glycine max | |
additional information | activity is independent of free Mg2+ concentration in the range 4-20 mM | Ricinus communis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
4 * 56000, gel filtration | Glycine max |
56000 | - |
4 * 56000, gel filtration | Ricinus communis |
230000 | - |
gel filtration | Glycine max |
230000 | - |
gel filtration | Ricinus communis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Ricinus communis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Butyl-Sepharose chromatography and Fractogel EMD DEAE-650 (S) anion-exchange chromatography | Ricinus communis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Ricinus communis | - |
embryo | - |
Glycine max | - |
endosperm | developing endosperm | Ricinus communis | - |
seed | - |
Glycine max | - |
seed | - |
Ricinus communis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.039 | - |
clarified extract | Ricinus communis |
73.1 | - |
after 1874fold purification | Ricinus communis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | - |
Glycine max | ATP + pyruvate | - |
? | |
ADP + phosphoenolpyruvate | - |
Ricinus communis | ATP + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 56000, gel filtration | Glycine max |
homotetramer | 4 * 56000, gel filtration | Ricinus communis |
Synonyms | Comment | Organism |
---|---|---|
PKC | - |
Glycine max |
PKC | - |
Ricinus communis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | 7.4 | displays a broad pH optimum of 6.4, however, more efficient substrate utilization is observed at pH 7.4 | Ricinus communis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.25 | - |
Ricinus communis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Glycine max | |
ADP | - |
Ricinus communis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1.2 | - |
IC50: 2.5 mM at pH 6.4, IC50: 1.2 mM at pH 7.4 | Ricinus communis | glutamate | |
8 | - |
IC50: 8.4 mM at pH 6.4, IC50: 8.0 mM at pH 7.4 | Ricinus communis | D-fructose 1,6-bisphosphate | |
13.7 | - |
IC50: 8.8 mM at pH 6.4, IC50: 13.7 mM at pH 7.4 | Ricinus communis | ATP | |
14.2 | - |
IC50: 9.2 mM at pH 6.4, IC50: 14.2 mM at pH 7.4 | Ricinus communis | citrate | |
17.2 | - |
IC50: 9.8 mM at pH 6.4, IC50: 17.2 mM at pH 7.4 | Ricinus communis | diphosphate |