Information on EC 2.7.7.53 - ATP adenylyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.7.53
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RECOMMENDED NAME
GeneOntology No.
ATP adenylyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ADP + ATP = phosphate + P1,P4-bis(5'-adenosyl) tetraphosphate
show the reaction diagram
GTP and adenosine can also act as adenylyl acceptors
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-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Purine metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
ADP:ATP adenylyltransferase
GTP and adenosine tetraphosphate can also act as adenylyl acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
96697-71-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
green alga
-
-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
strain CGY 339
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Manually annotated by BRENDA team
green alga
-
-
Manually annotated by BRENDA team
green alga
-
-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
green alga
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + arsenate
AMP + phosphate + ?
show the reaction diagram
-
-
-
?
ADP + ATP
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
show the reaction diagram
ADP + phosphate
phosphate + ADP
show the reaction diagram
NDP + arsenate
NMP + phosphate + ?
show the reaction diagram
NDP + phosphate
phosphate + NDP
show the reaction diagram
NDP + polyphosphate
polyphosphate + NDP
show the reaction diagram
-
exchange reaction
-
r
NTP + N'DP
P1,P4-bis(5'-nucleosyl) tetraphosphate' + phosphate
show the reaction diagram
P1(5'-adenosyl)-P4(5'-cytosyl) tetraphosphate + phosphate
CDP + ATP
show the reaction diagram
-
-
not CTP + ADP
?
P1(5'-adenosyl)-P4(5'-guanosyl) tetraphosphate + phosphate
GDP + ATP
show the reaction diagram
-
-
-
r
P1,P2-bis(5'-adenosyl) diphosphate + phosphate
?
show the reaction diagram
P1,P3-bis(5'-adenosyl) hexaphosphate + phosphate
?
show the reaction diagram
P1,P3-bis(5'-adenosyl) pentaphosphate + phosphate
?
show the reaction diagram
P1,P3-bis(5'-adenosyl) tetraphosphate + phosphate
ADP + ATP
show the reaction diagram
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
2 ADP
show the reaction diagram
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
ADP
show the reaction diagram
P1,P3-bis(5'-adenosyl) triphosphate with CF2-bridge between P1 and P2 + phosphate
?
show the reaction diagram
P1,P3-bis(5'-adenosyl) triphosphate with CH2-bridge between P1 and P2 + phosphate
?
show the reaction diagram
P1,P3-bis(5'-adenosyl) triphosphate with CHF-bridge between P1 and P2 + phosphate
?
show the reaction diagram
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
?
show the reaction diagram
P1,P4-bis(5'-adenosyl) tetraphosphate + chromate
?
show the reaction diagram
P1,P4-bis(5'-adenosyl) tetraphosphate + molybdate
?
show the reaction diagram
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
?
show the reaction diagram
-
involved in catabolism of dinucleoside polyphosphates
-
-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
ADP + ATP
show the reaction diagram
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
ATP + ADP
show the reaction diagram
P1,P4-bis(5'-adenosyl) tetraphosphate + tungstate
?
show the reaction diagram
P1,P4-bis(5'-adenosyl) tetraphosphate + vanadate
?
show the reaction diagram
P1,P4-bis(5'-adenosyl) tetraphosphate with CH2-bridge between P3 and P4 + phosphate
?
show the reaction diagram
P1,P4-bis(5'-guanosyl) pentaphosphate + phosphate
?
show the reaction diagram
-
wild-type, 106% of the rate with P1,P3-bis(5'-adenosyl) tetraphosphate, mutant A149Del 95%, respectively
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-
?
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
GDP + GTP
show the reaction diagram
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
GTP + GDP
show the reaction diagram
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
?
show the reaction diagram
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
adenosine tetraphosphate + ADP
show the reaction diagram
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
ADP + p4A
show the reaction diagram
P1,P5-bis(5'-guanosyl) pentaphosphate + phosphate
guanosine tetraphosphate + GDP
show the reaction diagram
106% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate
-
-
?
P1,P5-bis(5'-guanosyl) pentaphosphate + phosphate
p4G + GDP
show the reaction diagram
P1,P6-bis(5'-adenosyl) hexaphosphate + phosphate
adenosine pentaphosphate + ADP
show the reaction diagram
25% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate
-
-
?
P1-(5'-adenosyl)-P4-(5'-deoxythymidyl) tetraphosphate + phosphate
ATP + dTDP
show the reaction diagram
68% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
or ADP + dTTP, major products
-
?
P1-(5'-adenosyl)-P5-(5'-deoxythymidyl) pentaphosphate + phosphate
p4A + dTDP
show the reaction diagram
30% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
or ADP + p4dT, major products
-
?
P1-(5'-adenosyl)-P5-(5'-guanosyl) pentaphosphate + phosphate
p4A + GDP
show the reaction diagram
49% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
major products
-
?
P1-(5'-adenosyl)P4-(5'-deoxythymidyl) pentaphosphate + phosphate
p4A + dTDP
show the reaction diagram
31% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
or ADP + p4dT, major products
-
?
P1-(5'-adenosyl)P4-(5'-deoxythymidyl) tetraphosphate + phosphate
ATP + dTDP
show the reaction diagram
85% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
or ADP + dTTP, major products
-
?
P1-(5'-adenosyl)P4-(5'-guanosyl) pentaphosphate + phosphate
p4A + GDP
show the reaction diagram
30% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
major products
-
?
P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate
ATP + GDP
show the reaction diagram
P1-(5'-adenosyl)P5-(5'-guanosyl) pentaphosphate + phosphate
adenosine tetraphosphate + GDP
show the reaction diagram
76% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate
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-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
?
show the reaction diagram
-
involved in catabolism of dinucleoside polyphosphates
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Divalent metal cations
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AMP
-
ADP/phosphate-exchange
Ap3A
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ADP/phosphate-exchange
Ca2+
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weak, NDP-arsenolysis or NDP/phosphate-exchange reaction
Cd2+
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only NDP-arsenolysis or NDP/phosphate-exchange reaction
Co2+
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weak, NDP-arsenolysis or NDP/phosphate-exchange reaction
dithioerythritol
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-
Mn2+
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weak, NDP-arsenolysis or NDP/phosphate-exchange reaction
N-ethylmaleimide
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p-hydroxymercuribenzoate
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P1,P3-bis(5'-adenosyl) triphosphate with CCl2-bridge between P1 and P2
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i.e. ApCCl2ppA
P1,P3-bis(5'-adenosyl) triphosphate with CHCl-bridge between P1 and P2
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i.e. ApCHClppA
P1,P4-bis(5'-adenosyl) tetraphosphate
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ADP/phosphate-exchange
additional information
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no inhibition by F- or adenosine 5'-tetraphosphate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.31 - 0.7
ADP
0.13 - 3
arsenate
5.7
ATP
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pH 5.9, 37°C
0.52
chromate
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P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C
0.7
GDP
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ADP/phosphate-exchange, pH 7.0, 37°C
0.45
molybdate
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P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C
0.009 - 0.025
P1,P3-bis(5'-adenosyl) triphosphate
0.004
P1,P3-bis(5'-adenosyl) triphosphate with CF2-bridge between P1 and P2
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pH 8.0, 30°C
0.012
P1,P3-bis(5'-adenosyl) triphosphate with CH2-bridge between P1 and P2
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pH 8.0, 30°C
0.0035
P1,P3-bis(5'-adenosyl) triphosphate with CHF-bridge between P1 and P2
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pH 8.0, 30°C
0.0053 - 0.11
P1,P4-bis(5'-adenosyl) tetraphosphate
0.16 - 2
phosphate
1.75
tungstate
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P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C
0.07
vanadate
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P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
96
ADP
Saccharomyces cerevisiae
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ADP/phosphate-exchange, pH 7.0, 37°C
0.48 - 8.48
P1,P4-bis(5'-adenosyl) tetraphosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.9 - 84.8
P1,P4-bis(5'-adenosyl) tetraphosphate
1417
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.014
P1,P3-bis(5'-adenosyl) triphosphate with CCl2-bridge between P1 and P2
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pH 8.0, 30°C
0.008
P1,P3-bis(5'-adenosyl) triphosphate with CHCl-bridge between P1 and P2
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pH 8.0, 30°C
additional information
additional information
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00014
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crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
0.00065
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crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
0.00096
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crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
0.0012
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crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
0.037
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partially purified enzyme
1.96
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purified enzyme, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
3.01
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crude enzyme, using P1,P4-bis(5'-adenosyl) tetraphosphate as substrate, in 50 mM HEPES-Na (pH 7.6), 1 mM MnCl2, at 37°C
10.84
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after 3.6fold purification, using P1,P4-bis(5'-adenosyl) tetraphosphate as substrate, in 50 mM HEPES-Na (pH 7.6), 1 mM MnCl2, at 37°C
18.8
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purified enzyme
55
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.3
-
P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis assay at
5.9
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P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis
6.5
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UDP + phosphate, NDP/phosphate-exchange, ADP, GDP or CDP + arsenate, NDP-arsenolysis
7
-
ADP or CDP + phosphate, NDP/phosphate-exchange
additional information
-
pH-dependence of NDP/phosphate-exchange reaction
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
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about half-maximal activity at pH 6.5 and pH 9.0, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
6.5 - 9.8
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about 20% of maximal activity at pH 6.5 and about 80% of maximal activity at pH 9.8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24683
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4 * 24683, MALDI-TOF mass spectrometry
39000
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gel filtration
46000 - 48000
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gel filtration
46000
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1 * 46000, SDS-PAGE
100000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structure at 1.89 A resolution by using single-wavelength anomalous dispersion. Asn139, Gly146, and Ser147 are important active-site residues, Asn139 has a critical role in catalysis. The position of Gly146 might influence the phosphorylase activity. The tetrameric structure and the presence of Trp160 might be essential for the formation of the Ap4A binding site
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65
-
the activity completely disappears after treatment at 65°C for 10 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dithioerythritol stabilizes
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glycerol, 10%, stabilizes
-
most stable in Tris or phosphate buffered saline, 20 mM potassium phosphate, pH 6.8, NaCl-solution or 0.2 M glycine plus 0.1 mM EGTA, pH 2.7
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 30 mM HEPES/KOH buffer, pH 7.8, 50% glycerol, stable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
HisTrap HP column chromatography and Sephacryl S-200 gel filtration
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P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis: 2600fold, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis: 8531fold
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partial, 206fold
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3)pLysS cells
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expression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A149Del
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an Ala149 deletion mutant displays substrate specificity for diadenosine 5',5'''-P1,P5-pentaphosphate and is inactive on Ap4A and other substrates utilized by the wild-type enzyme
G146Q
-
about 20% of wild-type activity
G146S
-
about 30% of wild-type activity
N139A
-
complete loss of activity
N139Q
-
complete loss of activity
N139Q/S147T
-
complete loss of activity
S147A
-
about 8% of wild-type activity
S147T
-
about 15% of wild-type activity
W160A
-
complete loss of activity
A149Del
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an Ala149 deletion mutant displays substrate specificity for diadenosine 5',5'''-P1,P5-pentaphosphate and is inactive on Ap4A and other substrates utilized by the wild-type enzyme
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G146S
-
about 30% of wild-type activity
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N139A
-
complete loss of activity
-
N139Q
-
complete loss of activity
-
S147A
-
about 8% of wild-type activity
-
W160A
-
complete loss of activity
-
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