Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
crystal structure at 1.89 A resolution by using single-wavelength anomalous dispersion. Asn139, Gly146, and Ser147 are important active-site residues, Asn139 has a critical role in catalysis. The position of Gly146 might influence the phosphorylase activity. The tetrameric structure and the presence of Trp160 might be essential for the formation of the Ap4A binding site | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
G146Q | about 20% of wild-type activity | Mycobacterium tuberculosis |
G146S | about 30% of wild-type activity | Mycobacterium tuberculosis |
N139A | complete loss of activity | Mycobacterium tuberculosis |
N139Q | complete loss of activity | Mycobacterium tuberculosis |
N139Q/S147T | complete loss of activity | Mycobacterium tuberculosis |
S147A | about 8% of wild-type activity | Mycobacterium tuberculosis |
S147T | about 15% of wild-type activity | Mycobacterium tuberculosis |
W160A | complete loss of activity | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | mutant S147A, pH 7.6, 37°C | Mycobacterium tuberculosis | |
0.08 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | mutant G146Q, pH 7.6, 37°C | Mycobacterium tuberculosis | |
0.08 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | mutant G146S, pH 7.6, 37°C | Mycobacterium tuberculosis | |
0.1 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | wild-type, pH 7.6, 37°C | Mycobacterium tuberculosis | |
0.11 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | mutant S147T, pH 7.6, 37°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WMK9 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WMK9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
Mycobacterium tuberculosis | ADP + ATP | - |
? | |
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
Mycobacterium tuberculosis H37Rv | ADP + ATP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5',5'''-P1,P4-tetraphosphate phosphorylase | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | mutant S147A, pH 7.6, 37°C | Mycobacterium tuberculosis | |
1.38 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | mutant G146Q, pH 7.6, 37°C | Mycobacterium tuberculosis | |
1.49 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | mutant S147T, pH 7.6, 37°C | Mycobacterium tuberculosis | |
1.99 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | mutant G146S, pH 7.6, 37°C | Mycobacterium tuberculosis | |
8.48 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | wild-type, pH 7.6, 37°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.9 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | mutant S147A, pH 7.6, 37°C | Mycobacterium tuberculosis | |
13.6 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | mutant S147T, pH 7.6, 37°C | Mycobacterium tuberculosis | |
17.25 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | mutant G146Q, pH 7.6, 37°C | Mycobacterium tuberculosis | |
24.88 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | mutant G146S, pH 7.6, 37°C | Mycobacterium tuberculosis | |
84.8 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | wild-type, pH 7.6, 37°C | Mycobacterium tuberculosis |