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4-aminobutanoate + glyoxylate
succinate semialdehyde + glycine
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
glyoxylate + L-alanine
?
-
-
-
?
additional information
?
-
4-aminobutanoate + glyoxylate
succinate semialdehyde + glycine
-
-
-
ir
4-aminobutanoate + glyoxylate
succinate semialdehyde + glycine
the glyoxylate-dependent reaction is not reversible, although glyoxylate can also be used in conjunction with alanine as a substrate in the reverse reaction
-
-
ir
4-aminobutanoate + glyoxylate
succinate semialdehyde + glycine
-
-
-
ir
4-aminobutanoate + glyoxylate
succinate semialdehyde + glycine
-
-
-
ir
4-aminobutanoate + glyoxylate
succinate semialdehyde + glycine
-
-
-
?
4-aminobutanoate + glyoxylate
succinate semialdehyde + glycine
-
-
-
?
4-aminobutanoate + glyoxylate
succinate semialdehyde + glycine
-
-
-
?
4-aminobutanoate + glyoxylate
succinate semialdehyde + glycine
-
-
-
?
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
-
?
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
?
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
r
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
the pyruvate-dependent GABA-T reaction is reversible
-
-
r
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
?
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
-
?
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
-
r
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
pyruvate-specific mitochondrial GABA-T
-
-
r
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
-
r
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
pyruvate-specific mitochondrial GABA-T
-
-
r
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
?
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
?
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
?
additional information
?
-
-
no activity with 2-oxoglutarate
-
-
?
additional information
?
-
GABA-T catalyses the breakdown of GABA to succinic semialdehyde. The enzyme shows both pyruvate- and glyoxylate-dependent activities, but no activity with 2-oxoglutarate. The substrate specificity at pH 9 reveals that the enzyme utilizes glyoxylate, as well as pyruvate, as amino acceptors in the forward reaction, and GABA, but not beta-alanine, ornithine, acetylornithine, serine, glycine, asparagine, glutamine, glutamate, valine, leucine, isoleucine, methionine, phenylalanine, histidine, lysine, arginine, aspartate, threonine, tyrosine, tryptophan, proline, or cysteine as amino donors
-
-
?
additional information
?
-
GABA-T catalyses the breakdown of GABA to succinic semialdehyde. The enzyme shows both pyruvate- and glyoxylate-dependent activities, but no activity with 2-oxoglutarate. The substrate specificity at pH 9 reveals that the enzyme utilizes glyoxylate, as well as pyruvate, as amino acceptors in the forward reaction, and GABA, but not beta-alanine, ornithine, acetylornithine, serine, glycine, asparagine, glutamine, glutamate, valine, leucine, isoleucine, methionine, phenylalanine, histidine, lysine, arginine, aspartate, threonine, tyrosine, tryptophan, proline, or cysteine as amino donors
-
-
?
additional information
?
-
GABA-T catalyses the breakdown of GABA to succinic semialdehyde. The enzyme shows both pyruvate- and glyoxylate-dependent activities, but no activity with 2-oxoglutarate. The substrate specificity at pH 9 reveals that the enzyme utilizes glyoxylate, as well as pyruvate, as amino acceptors in the forward reaction, and GABA, but not beta-alanine, ornithine, acetylornithine, serine, glycine, asparagine, glutamine, glutamate, valine, leucine, isoleucine, methionine, phenylalanine, histidine, lysine, arginine, aspartate, threonine, tyrosine, tryptophan, proline, or cysteine as amino donors
-
-
?
additional information
?
-
enzyme utilizes pyruvate and glyoxylate, but not 2-oxoglutarate
-
-
?
additional information
?
-
-
no activity with 2-oxoglutarate
-
-
?
additional information
?
-
AK103274
no substrate: 2-oxoglutarate
-
-
?
additional information
?
-
no substrate: 2-oxoglutarate
-
-
?
additional information
?
-
no substrate: 2-oxoglutarate
-
-
?
additional information
?
-
GABA-T isozymes catalyse the breakdown of GABA to succinic semialdehyde. The isozymes all have both pyruvate- and glyoxylate-dependent activities, and utilize GABA and Ala but not beta-Ala, Orn, acetylornithine, Ser, Gly, Asn, Gln, Glu, Val, Leu, Ile, Met, Phe, His, Lys, Arg, Asp, Thr, Tyr, Trp, Pro, or Cys as amino donors
-
-
?
additional information
?
-
GABA-T isozymes catalyse the breakdown of GABA to succinic semialdehyde. The isozymes all have both pyruvate- and glyoxylate-dependent activities, and utilize GABA and Ala but not beta-Ala, Orn, acetylornithine, Ser, Gly, Asn, Gln, Glu, Val, Leu, Ile, Met, Phe, His, Lys, Arg, Asp, Thr, Tyr, Trp, Pro, or Cys as amino donors
-
-
?
additional information
?
-
GABA-T isozymes catalyse the breakdown of GABA to succinic semialdehyde. The isozymes all have both pyruvate- and glyoxylate-dependent activities, and utilize GABA and Ala but not beta-Ala, Orn, acetylornithine, Ser, Gly, Asn, Gln, Glu, Val, Leu, Ile, Met, Phe, His, Lys, Arg, Asp, Thr, Tyr, Trp, Pro, or Cys as amino donors
-
-
?
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4-aminobutanoate + glyoxylate
succinate semialdehyde + glycine
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
4-aminobutanoate + glyoxylate
succinate semialdehyde + glycine
-
-
-
ir
4-aminobutanoate + glyoxylate
succinate semialdehyde + glycine
-
-
-
ir
4-aminobutanoate + glyoxylate
succinate semialdehyde + glycine
-
-
-
ir
4-aminobutanoate + glyoxylate
succinate semialdehyde + glycine
-
-
-
?
4-aminobutanoate + glyoxylate
succinate semialdehyde + glycine
-
-
-
?
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
-
?
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
?
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
r
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
-
?
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
-
r
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
-
r
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
?
4-aminobutanoate + pyruvate
succinate semialdehyde + L-alanine
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.0004
crude enzyme extract, substrate pyruvate, pH 9.0, 30°C
0.0005
substrate glyoxylate, pH 9.0, 30°C
0.0013
substrate pyruvate, pH 9.0, 30°C
0.0183
purified recombinant isozyme GABA-T2, with pyruvate as substrate, pH 9.0, 30°C
0.0209
purified recombinant isozyme GABA-T2, with glyoxylate as substrate, pH 9.0, 30°C
0.052
substrate pyruvate, pH 9.0, 30°C
0.066
substrate glyoxylate, pH 9.0, 30°C
0.069
substrate glyoxylate, pH 9.0, 30°C
0.17
-
purified enzyme, pH 8.2, temperature not specified in the publication
0.179
purified recombinant isozyme GABA-T3, with glyoxylate as substrate, pH 9.0, 30°C
3.5
purified recombinant isozyme GABA-T1, with glyoxylate as substrate, pH 9.0, 30°C
3.53
purified recombinant full-length enzyme, substrate pyruvate, pH 9.0, 30°C
5.79
purified recombinant isozyme GABA-T1, with pyruvate as substrate, pH 9.0, 30°C
7.54
purified recombinant truncated enzyme, substrate pyruvate, pH 9.0, 30°C
0.207
substrate pyruvate, pH 9.0, 30°C
0.207
purified recombinant isozyme GABA-T3, with pyruvate as substrate, pH 9.0, 30°C
additional information
native GABA-T activity in cell-free extracts of Arabidopsis thaliana ecotype Columbia and corresponding knockout mutant, overview
additional information
native GABA-T activity in cell-free extracts of Arabidopsis thaliana ecotype Wassilewskija and corresponding knockout mutant, overview
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GABA-T1 is the predominant isoform in stamens and maturing fruit tissue
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expression is significantly upregulated in youngest leaves
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transcription is significantly upregulated in the youngest leaves
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green leaves at the beginning of flowering
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expression is slightly upregulated in veins and petioles compared to leaf veins
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expression is slightly upregulated in veins and petioles compared to leaf veins
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highest expression
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least expression
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additional information
BnaA.GABA-T.a is the least expressed of the enzyme isoforms, except in roots
brenda
additional information
BnaA.GABA-T.a is the least expressed of the enzyme isoforms, except in roots
brenda
additional information
BnaA.GABA-T.a is the least expressed of the enzyme isoforms, except in roots
brenda
additional information
BnaA.GABA-T.a is the least expressed of the enzyme isoforms, except in roots
brenda
additional information
expression is slightly upregulated in veins and petioles compared to leaf veins
brenda
additional information
expression is slightly upregulated in veins and petioles compared to leaf veins
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additional information
expression is slightly upregulated in veins and petioles compared to leaf veins
brenda
additional information
expression is slightly upregulated in veins and petioles compared to leaf veins
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additional information
isoform GABA-T is the predominantly expressed form
brenda
additional information
isoform GABA-T is the predominantly expressed form
brenda
additional information
isoform GABA-T is the predominantly expressed form
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additional information
isoform GABA-T is the predominantly expressed form
brenda
additional information
-
two of the four genes are more highly expressed in the vegetative organs tested, but exhibit a different pattern during seed maturation
brenda
additional information
two of the four genes are more highly expressed in the vegetative organs tested, but exhibit a different pattern during seed maturation
brenda
additional information
two of the four genes are more highly expressed in the vegetative organs tested, but exhibit a different pattern during seed maturation
brenda
additional information
two of the four genes are more highly expressed in the vegetative organs tested, but exhibit a different pattern during seed maturation
brenda
additional information
differential expression patterns of the three GABA-T isoforms in reproductive tissues, but not vegetative tissues, overview
brenda
additional information
differential expression patterns of the three GABA-T isoforms in reproductive tissues, but not vegetative tissues, overview
brenda
additional information
differential expression patterns of the three GABA-T isoforms in reproductive tissues, but not vegetative tissues, overview
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli
gene GABA-T1, sequencce comparisons, expression of isozyme GABA-T1 in Escherichia coli strain BL21 (DE3), transient expression of fluorescence-labeled GABA-T proteins in onion epidermal cells, two of the four enzymes are targeted to mitochondria, a third to chloroplasts, and the fourth to cytosol
gene GABA-T2, sequencce comparisons, expression of isozyme GABA-T2 in Escherichia coli strain BL21 (DE3), transient expression of fluorescence-labeled GABA-T proteins in onion epidermal cells, two of the four enzymes are targeted to mitochondria, a third to chloroplasts, and the fourth to cytosol
gene GABA-T3, sequence comparisons, expression of isozyme GABA-T3 in Escherichia coli strain BL21 (DE3), transient expression of fluorescence-labeled GABA-T proteins in onion epidermal cells, two of the four enzymes are targeted to mitochondria, a third to chloroplasts, and the fourth to cytosol
gene GABA-T4, sequencce comparisons, expression of isozyme GABA-T4 in Escherichia coli strain BL21 (DE3), transient expression of fluorescence-labeled GABA-T proteins in onion epidermal cells, two of the four enzymes are targeted to mitochondria, a third to chloroplasts, and the fourth to cytosol
gene pop2, expression of N-termminally His6-tagged GABA-T POP2 in Escherichia coli strain BL-21(DE3) Rosetta (pLysS)
genes pop2-1, pop2-2, and pop2-3, pop2-1 are located on chromosome 3, DNA and amino acid sequennce determination and analysis
isozyme GABA-T1, DNA and amino acid sequence determination and analysis, expression of His-tagged GABA-T1 in Escherichia coli strain BL-21(DE3) Rosetta (pLysS), expression and subcellular localization of tomato GABA-T isozymes in Nicotiana tabacum BY-2 suspension-cultured cells
isozyme GABA-T2, DNA and amino acid sequence determination and analysis, expression of His-tagged GABA-T2 in Escherichia coli strain BL-21(DE3) Rosetta (pLysS), expression and subcellular localization of tomato GABA-T isozymes in Nicotiana tabacum BY-2 suspension-cultured cells
isozyme GABA-T3, DNA and amino acid sequence determination and analysis, expression of His-tagged GABA-T3 in Escherichia coli strain BL-21(DE3) Rosetta (pLysS), expression and subcellular localization of tomato GABA-T isozymes in Nicotiana tabacum BY-2 suspension-cultured cells
removal of the N-terminal targeting presequences yields good recovery of the soluble recombinant proteins in Escherichia coli when they are co-expressed with the GroES/EL molecular chaperone complex
transient expression of GABA-T in Nicotiana tabacum BY-2 cells. C-terminal myc-tagged GABA-T co-localizes with coexpressed mitochondrial betaATPase-CAT in BY-2 cells
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli
-
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli
-
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Van Cauwenberghe, O.R.; Shelp, B.J.
Biochemical characterization of partially purified gaba:pyruvate transaminase from Nicotiana tabacum
Phytochemistry
52
575-581
1999
Nicotiana tabacum, Nicotiana tabacum Samsun N.N.
-
brenda
Clark, S.M.; Di Leo, R.; Dhanoa, P.K.; Van Cauwenberghe, O.R.; Mullen, R.T.; Shelp, B.J.
Biochemical characterization, mitochondrial localization, expression, and potential functions for an Arabidopsis gamma-aminobutyrate transaminase that utilizes both pyruvate and glyoxylate
J. Exp. Bot.
60
1743-1757
2009
Arabidopsis thaliana (Q94CE5), Arabidopsis thaliana Wassilewskija (Q94CE5), Arabidopsis thaliana Col-0 (Q94CE5)
brenda
Clark, S.M.; Di Leo, R.; Van Cauwenberghe, O.R.; Mullen, R.T.; Shelp, B.J.
Subcellular localization and expression of multiple tomato gamma-aminobutyrate transaminases that utilize both pyruvate and glyoxylate
J. Exp. Bot.
60
3255-3267
2009
Solanum lycopersicum (Q84P52), Solanum lycopersicum (Q84P53), Solanum lycopersicum (Q84P54)
brenda
Van Cauwenberghe, O.; Makhmoudova, A.; McLean, M.; Clark, S.; Shelp, B.
Plant pyruvate-dependent gamma-aminobutyrate transaminase: Identification of an Arabidopsis cDNA and its expression in Escherichia coli
Can. J. Bot.
80
933-941
2002
Arabidopsis thaliana, Nicotiana tabacum
brenda
Palanivelu, R.; Brass, L.; Edlund, A.; Preuss, D.
Pollen tube growth and guidance is regulated by POP2, an Arabidopsis gene that controls GABA levels
Cell
114
47-59
2003
Arabidopsis thaliana (Q94CE5)
brenda
Shimajiri, Y.; Ozaki, K.; Kainou, K.; Akama, K.
Differential subcellular localization, enzymatic properties and expression patterns of gamma-aminobutyric acid transaminases (GABA-Ts) in rice (Oryza sativa)
J. Plant Physiol.
170
196-201
2013
Oryza sativa, Oryza sativa (Q6ZH29), Oryza sativa (Q7XN11), Oryza sativa (Q7XN12)
brenda
Trobacher, C.; Clark, S.; Bozzo, G.; Mullen, R.; DeEll, J.; Shelp, B.
Catabolism of GABA in apple fruit: Subcellular localization and biochemical characterization of two gamma-aminobutyrate transaminases
Postharvest Biol. Technol.
75
106-113
2013
Malus domestica (J9XGZ5)
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brenda
Faes, P.; Niogret, M.; Montes, E.; Caherec, F.; Bouchereau, A.; Deleu, C.
Transcriptional profiling of genes encoding GABA-transaminases in Brassica napus reveals their regulation by water deficit
Environ. Exp. Bot.
116
20-31
2015
Brassica napus (A0A078FCE5), Brassica napus (A0A078HJ76), Brassica napus (A0A078HPK5), Brassica napus (A0A0H4AKW3)
-
brenda
Shimajiri, Y.; Oonishi, T.; Ozaki, K.; Kainou, K.; Akama, K.
Genetic manipulation of the gamma-aminobutyric acid (GABA) shunt in rice: overexpression of truncated glutamate decarboxylase (GAD2) and knockdown of gamma-aminobutyric acid transaminase (GABA-T) lead to sustained and high levels of GABA accumulation in rice kern
Plant Biotechnol. J.
11
594-604
2013
Oryza sativa (AK103274), Oryza sativa (Q7XN11), Oryza sativa (Q7XN12)
brenda
Deleu, C.; Faes, P.; Niogret, M.F.; Bouchereau, A.
Effects of the inhibitor of the gamma-aminobutyrate-transaminase, vinyl-gamma-aminobutyrate, on development and nitrogen metabolism in Brassica napus seedlings
Plant Physiol. Biochem.
64
60-69
2013
Brassica napus
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