Any feedback?
Literature summary for 2.6.1.96 extracted from
- Biochemical characterization of partially purified gaba:pyruvate transaminase from Nicotiana tabacum (1999), Phytochemistry, 52, 575-581.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aminooxyacetic acid | 80% inhibition at 2 mM | Nicotiana tabacum |  |
| beta-Alanine | 30% inhibition at 2 mM | Nicotiana tabacum | |
| gabaculine | 80% inhibition at 2 mM | Nicotiana tabacum | |
| L-glutamine | 11% inhibition at 2 mM | Nicotiana tabacum | |
| L-ornithine | 40% inhibition at 2 mM | Nicotiana tabacum | |
| additional information | no or poor inhibition by proline and succinate | Nicotiana tabacum | |
| Succinic semialdehyde | 67% inhibition at 2 mM | Nicotiana tabacum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.24 | - |
pyruvate | pH 8.2, temperature not specified in the publication | Nicotiana tabacum | |
| 1.2 | - |
4-aminobutanoate | pH 8.2, temperature not specified in the publication | Nicotiana tabacum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Nicotiana tabacum | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-aminobutanoate + pyruvate | Nicotiana tabacum | - |
succinate semialdehyde + L-alanine | - |
r | |
| 4-aminobutanoate + pyruvate | Nicotiana tabacum Samsun N.N. | - |
succinate semialdehyde + L-alanine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana tabacum | - |
- |
- |
| Nicotiana tabacum Samsun N.N. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| the pyruvate-dependent GABA-T activity is separated from 2-oxoglutarate-dependent GABA-T activity, EC 2.6.1.19, by two steps of anion exchange chromatography, purification 1530fold | Nicotiana tabacum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Nicotiana tabacum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.17 | - |
purified enzyme, pH 8.2, temperature not specified in the publication | Nicotiana tabacum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-aminobutanoate + pyruvate | - |
Nicotiana tabacum | succinate semialdehyde + L-alanine | - |
r | |
| 4-aminobutanoate + pyruvate | pyruvate-specific mitochondrial GABA-T | Nicotiana tabacum | succinate semialdehyde + L-alanine | - |
r | |
| 4-aminobutanoate + pyruvate | - |
Nicotiana tabacum Samsun N.N. | succinate semialdehyde + L-alanine | - |
r | |
| 4-aminobutanoate + pyruvate | pyruvate-specific mitochondrial GABA-T | Nicotiana tabacum Samsun N.N. | succinate semialdehyde + L-alanine | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4-aminobutyrate transaminase | - |
Nicotiana tabacum |
| gaba:pyruvate transaminase | - |
Nicotiana tabacum |
| pyruvate-specific mitochondrial GABA-T | - |
Nicotiana tabacum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
assay at | Nicotiana tabacum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Nicotiana tabacum | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
