Cloned (Comment) | Organism |
---|---|
isozyme GABA-T1, DNA and amino acid sequence determination and analysis, expression of His-tagged GABA-T1 in Escherichia coli strain BL-21(DE3) Rosetta (pLysS), expression and subcellular localization of tomato GABA-T isozymes in Nicotiana tabacum BY-2 suspension-cultured cells | Solanum lycopersicum |
isozyme GABA-T2, DNA and amino acid sequence determination and analysis, expression of His-tagged GABA-T2 in Escherichia coli strain BL-21(DE3) Rosetta (pLysS), expression and subcellular localization of tomato GABA-T isozymes in Nicotiana tabacum BY-2 suspension-cultured cells | Solanum lycopersicum |
isozyme GABA-T3, DNA and amino acid sequence determination and analysis, expression of His-tagged GABA-T3 in Escherichia coli strain BL-21(DE3) Rosetta (pLysS), expression and subcellular localization of tomato GABA-T isozymes in Nicotiana tabacum BY-2 suspension-cultured cells | Solanum lycopersicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Solanum lycopersicum | 5739 | - |
additional information | the subcellular distribution of GABA-T in the plant kingdom is highly variable, in silico analysis of GABA-T isozyme subcellular localizations in tomato, overview | Solanum lycopersicum | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanoate + glyoxylate | Solanum lycopersicum | - |
succinate semialdehyde + glycine | - |
? | |
4-aminobutanoate + pyruvate | Solanum lycopersicum | - |
succinate semialdehyde + L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | Q84P52 | isozyme GABA-T3; contains three GABA-T isoforms | - |
Solanum lycopersicum | Q84P53 | isozyme GABA-T2; contains three GABA-T isoforms | - |
Solanum lycopersicum | Q84P54 | isozyme GABA-T1; contains three GABA-T isoforms | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozyme GABA-T1 from Escherichia coli strain BL-21(DE3) Rosetta (pLysS) by nickel affinity chromatgraphy | Solanum lycopersicum |
recombinant His-tagged isozyme GABA-T2 from Escherichia coli strain BL-21(DE3) Rosetta (pLysS) by nickel affinity chromatgraphy | Solanum lycopersicum |
recombinant His-tagged isozyme GABA-T3 from Escherichia coli strain BL-21(DE3) Rosetta (pLysS) by nickel affinity chromatgraphy | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
carpel | - |
Solanum lycopersicum | - |
fruit | - |
Solanum lycopersicum | - |
fruit | GABA-T1 is the predominant isoform in stamens and maturing fruit tissue | Solanum lycopersicum | - |
leaf | - |
Solanum lycopersicum | - |
additional information | differential expression patterns of the three GABA-T isoforms in reproductive tissues, but not vegetative tissues, overview | Solanum lycopersicum | - |
petal | - |
Solanum lycopersicum | - |
root | - |
Solanum lycopersicum | - |
sepal | - |
Solanum lycopersicum | - |
stamen | - |
Solanum lycopersicum | - |
stem | - |
Solanum lycopersicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0183 | - |
purified recombinant isozyme GABA-T2, with pyruvate as substrate, pH 9.0, 30°C | Solanum lycopersicum |
0.0209 | - |
purified recombinant isozyme GABA-T2, with glyoxylate as substrate, pH 9.0, 30°C | Solanum lycopersicum |
0.179 | - |
purified recombinant isozyme GABA-T3, with glyoxylate as substrate, pH 9.0, 30°C | Solanum lycopersicum |
0.207 | - |
purified recombinant isozyme GABA-T3, with pyruvate as substrate, pH 9.0, 30°C | Solanum lycopersicum |
3.5 | - |
purified recombinant isozyme GABA-T1, with glyoxylate as substrate, pH 9.0, 30°C | Solanum lycopersicum |
5.79 | - |
purified recombinant isozyme GABA-T1, with pyruvate as substrate, pH 9.0, 30°C | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanoate + glyoxylate | - |
Solanum lycopersicum | succinate semialdehyde + glycine | - |
? | |
4-aminobutanoate + pyruvate | - |
Solanum lycopersicum | succinate semialdehyde + L-alanine | - |
? | |
additional information | GABA-T isozymes catalyse the breakdown of GABA to succinic semialdehyde. The isozymes all have both pyruvate- and glyoxylate-dependent activities, and utilize GABA and Ala but not beta-Ala, Orn, acetylornithine, Ser, Gly, Asn, Gln, Glu, Val, Leu, Ile, Met, Phe, His, Lys, Arg, Asp, Thr, Tyr, Trp, Pro, or Cys as amino donors | Solanum lycopersicum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GABA-T | - |
Solanum lycopersicum |
gamma-aminobutyrate transaminase | - |
Solanum lycopersicum |
gamma-aminobutyric acid transaminase | - |
Solanum lycopersicum |
gammaaAminobutyric acid transaminase | - |
Solanum lycopersicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Solanum lycopersicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Solanum lycopersicum |
General Information | Comment | Organism |
---|---|---|
physiological function | unique roles for each isozyme in developmental processes | Solanum lycopersicum |