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Literature summary for 2.6.1.96 extracted from

  • Clark, S.M.; Di Leo, R.; Van Cauwenberghe, O.R.; Mullen, R.T.; Shelp, B.J.
    Subcellular localization and expression of multiple tomato gamma-aminobutyrate transaminases that utilize both pyruvate and glyoxylate (2009), J. Exp. Bot., 60, 3255-3267.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
isozyme GABA-T1, DNA and amino acid sequence determination and analysis, expression of His-tagged GABA-T1 in Escherichia coli strain BL-21(DE3) Rosetta (pLysS), expression and subcellular localization of tomato GABA-T isozymes in Nicotiana tabacum BY-2 suspension-cultured cells Solanum lycopersicum
isozyme GABA-T2, DNA and amino acid sequence determination and analysis, expression of His-tagged GABA-T2 in Escherichia coli strain BL-21(DE3) Rosetta (pLysS), expression and subcellular localization of tomato GABA-T isozymes in Nicotiana tabacum BY-2 suspension-cultured cells Solanum lycopersicum
isozyme GABA-T3, DNA and amino acid sequence determination and analysis, expression of His-tagged GABA-T3 in Escherichia coli strain BL-21(DE3) Rosetta (pLysS), expression and subcellular localization of tomato GABA-T isozymes in Nicotiana tabacum BY-2 suspension-cultured cells Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Solanum lycopersicum 5739
-
additional information the subcellular distribution of GABA-T in the plant kingdom is highly variable, in silico analysis of GABA-T isozyme subcellular localizations in tomato, overview Solanum lycopersicum
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-aminobutanoate + glyoxylate Solanum lycopersicum
-
succinate semialdehyde + glycine
-
?
4-aminobutanoate + pyruvate Solanum lycopersicum
-
succinate semialdehyde + L-alanine
-
?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum Q84P52 isozyme GABA-T3; contains three GABA-T isoforms
-
Solanum lycopersicum Q84P53 isozyme GABA-T2; contains three GABA-T isoforms
-
Solanum lycopersicum Q84P54 isozyme GABA-T1; contains three GABA-T isoforms
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme GABA-T1 from Escherichia coli strain BL-21(DE3) Rosetta (pLysS) by nickel affinity chromatgraphy Solanum lycopersicum
recombinant His-tagged isozyme GABA-T2 from Escherichia coli strain BL-21(DE3) Rosetta (pLysS) by nickel affinity chromatgraphy Solanum lycopersicum
recombinant His-tagged isozyme GABA-T3 from Escherichia coli strain BL-21(DE3) Rosetta (pLysS) by nickel affinity chromatgraphy Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
carpel
-
Solanum lycopersicum
-
fruit
-
Solanum lycopersicum
-
fruit GABA-T1 is the predominant isoform in stamens and maturing fruit tissue Solanum lycopersicum
-
leaf
-
Solanum lycopersicum
-
additional information differential expression patterns of the three GABA-T isoforms in reproductive tissues, but not vegetative tissues, overview Solanum lycopersicum
-
petal
-
Solanum lycopersicum
-
root
-
Solanum lycopersicum
-
sepal
-
Solanum lycopersicum
-
stamen
-
Solanum lycopersicum
-
stem
-
Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0183
-
purified recombinant isozyme GABA-T2, with pyruvate as substrate, pH 9.0, 30°C Solanum lycopersicum
0.0209
-
purified recombinant isozyme GABA-T2, with glyoxylate as substrate, pH 9.0, 30°C Solanum lycopersicum
0.179
-
purified recombinant isozyme GABA-T3, with glyoxylate as substrate, pH 9.0, 30°C Solanum lycopersicum
0.207
-
purified recombinant isozyme GABA-T3, with pyruvate as substrate, pH 9.0, 30°C Solanum lycopersicum
3.5
-
purified recombinant isozyme GABA-T1, with glyoxylate as substrate, pH 9.0, 30°C Solanum lycopersicum
5.79
-
purified recombinant isozyme GABA-T1, with pyruvate as substrate, pH 9.0, 30°C Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutanoate + glyoxylate
-
Solanum lycopersicum succinate semialdehyde + glycine
-
?
4-aminobutanoate + pyruvate
-
Solanum lycopersicum succinate semialdehyde + L-alanine
-
?
additional information GABA-T isozymes catalyse the breakdown of GABA to succinic semialdehyde. The isozymes all have both pyruvate- and glyoxylate-dependent activities, and utilize GABA and Ala but not beta-Ala, Orn, acetylornithine, Ser, Gly, Asn, Gln, Glu, Val, Leu, Ile, Met, Phe, His, Lys, Arg, Asp, Thr, Tyr, Trp, Pro, or Cys as amino donors Solanum lycopersicum ?
-
?

Synonyms

Synonyms Comment Organism
GABA-T
-
Solanum lycopersicum
gamma-aminobutyrate transaminase
-
Solanum lycopersicum
gamma-aminobutyric acid transaminase
-
Solanum lycopersicum
gammaaAminobutyric acid transaminase
-
Solanum lycopersicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Solanum lycopersicum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Solanum lycopersicum

General Information

General Information Comment Organism
physiological function unique roles for each isozyme in developmental processes Solanum lycopersicum