Cloned (Comment) | Organism |
---|---|
removal of the N-terminal targeting presequences yields good recovery of the soluble recombinant proteins in Escherichia coli when they are co-expressed with the GroES/EL molecular chaperone complex | Malus domestica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aminooxyacetate | effective inhibition at 250 microM | Malus domestica | |
beta-Alanine | 4365% inhibition at 1 mM | Malus domestica | |
gabaculine | i.e. 5-amino-1,3-cyclohexadienylcarboxylate, effective inhibition at 250 microM | Malus domestica | |
ornithine | 4365% inhibition at 1 mM | Malus domestica | |
vigabatrin | 93% inhibition at 1 mM | Malus domestica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
glyoxylate | pH 9.0, 25°C | Malus domestica | |
0.019 | - |
pyruvate | pH 9.0, 25°C | Malus domestica | |
0.077 | - |
4-aminobutanoate | cosubstrate pyruvate, pH 9.0, 25°C | Malus domestica | |
0.1 | - |
4-aminobutanoate | cosubstrate glyoxylate, pH 9.0, 25°C | Malus domestica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Malus domestica | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | J9XGZ5 | Borkh. cv. Empire | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanoate + glyoxylate | - |
Malus domestica | succinate semialdehyde + glycine | - |
? | |
4-aminobutanoate + pyruvate | - |
Malus domestica | succinate semialdehyde + L-alanine | - |
? | |
additional information | enzyme utilizes pyruvate and glyoxylate, but not 2-oxoglutarate | Malus domestica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GABA-T2 | - |
Malus domestica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.74 | - |
glyoxylate | pH 9.0, 25°C | Malus domestica | |
0.8 | - |
pyruvate | pH 9.0, 25°C | Malus domestica | |
0.84 | - |
4-aminobutanoate | cosubstrate pyruvate, pH 9.0, 25°C | Malus domestica | |
1 | - |
4-aminobutanoate | cosubstrate glyoxylate, pH 9.0, 25°C | Malus domestica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.5 | - |
Malus domestica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.4 | - |
4-aminobutanoate | cosubstrate glyoxylate, pH 9.0, 25°C | Malus domestica | |
10.9 | - |
4-aminobutanoate | cosubstrate pyruvate, pH 9.0, 25°C | Malus domestica | |
42.7 | - |
pyruvate | pH 9.0, 25°C | Malus domestica | |
45.6 | - |
glyoxylate | pH 9.0, 25°C | Malus domestica |