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Literature summary for 2.6.1.96 extracted from

  • Trobacher, C.; Clark, S.; Bozzo, G.; Mullen, R.; DeEll, J.; Shelp, B.
    Catabolism of GABA in apple fruit: Subcellular localization and biochemical characterization of two gamma-aminobutyrate transaminases (2013), Postharvest Biol. Technol., 75, 106-113.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
removal of the N-terminal targeting presequences yields good recovery of the soluble recombinant proteins in Escherichia coli when they are co-expressed with the GroES/EL molecular chaperone complex Malus domestica

Inhibitors

Inhibitors Comment Organism Structure
Aminooxyacetate effective inhibition at 250 microM Malus domestica
beta-Alanine 43–65% inhibition at 1 mM Malus domestica
gabaculine i.e. 5-amino-1,3-cyclohexadienylcarboxylate, effective inhibition at 250 microM Malus domestica
ornithine 43–65% inhibition at 1 mM Malus domestica
vigabatrin 93% inhibition at 1 mM Malus domestica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
glyoxylate pH 9.0, 25°C Malus domestica
0.019
-
pyruvate pH 9.0, 25°C Malus domestica
0.077
-
4-aminobutanoate cosubstrate pyruvate, pH 9.0, 25°C Malus domestica
0.1
-
4-aminobutanoate cosubstrate glyoxylate, pH 9.0, 25°C Malus domestica

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Malus domestica 5739
-

Organism

Organism UniProt Comment Textmining
Malus domestica J9XGZ5 Borkh. cv. Empire
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutanoate + glyoxylate
-
Malus domestica succinate semialdehyde + glycine
-
?
4-aminobutanoate + pyruvate
-
Malus domestica succinate semialdehyde + L-alanine
-
?
additional information enzyme utilizes pyruvate and glyoxylate, but not 2-oxoglutarate Malus domestica ?
-
?

Synonyms

Synonyms Comment Organism
GABA-T2
-
Malus domestica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.74
-
glyoxylate pH 9.0, 25°C Malus domestica
0.8
-
pyruvate pH 9.0, 25°C Malus domestica
0.84
-
4-aminobutanoate cosubstrate pyruvate, pH 9.0, 25°C Malus domestica
1
-
4-aminobutanoate cosubstrate glyoxylate, pH 9.0, 25°C Malus domestica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9.5
-
Malus domestica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10.4
-
4-aminobutanoate cosubstrate glyoxylate, pH 9.0, 25°C Malus domestica
10.9
-
4-aminobutanoate cosubstrate pyruvate, pH 9.0, 25°C Malus domestica
42.7
-
pyruvate pH 9.0, 25°C Malus domestica
45.6
-
glyoxylate pH 9.0, 25°C Malus domestica