Information on EC 2.4.1.252 - GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.252
-
RECOMMENDED NAME
GeneOntology No.
GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol = GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
acetan biosynthesis
-
-
xanthan biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
GDP-mannose:D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol 3-alpha-mannosyltransferase
In the bacterium Gluconacetobacter xylinus (previously known as Acetobacter xylinum) the enzyme is involved in the biosynthesis of the exopolysaccharide acetan [1]. In Xanthomonas campestris the enzyme is involved in the biosynthesis of the exopolysaccharide xanthan [5].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
pv. oryzae
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
additional information
-
the gum gene cluster constitutes an operon expressed from multiple promoters located upstream of gumB and gumG, respectively. The existence of two internal promoters is newly identified in the Xanthomonas oryzae gum gene cluster through the insertion mutations with the rrnB transcriptional terminator and RT-PCR analyses
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP-mannose + cellobiose-diphosphate-polyprenol carrier lipid
GDP + D-mannose-alpha-(1-3)-cellobiose-diphosphate-polyprenol carrier lipid
show the reaction diagram
-
GumH enzyme is an alpha-1,3-mannosyltransferase responsible for the transfer of mannose from GDP-mannose to the cellobiose-diphosphate-polyprenol carrier lipid during the assembly of the tetrasaccharide repeat unit of the exopolysaccharide fastidian gum
-
-
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
show the reaction diagram
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphophytanol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphophytanol
show the reaction diagram
-
synthetic phytanyl-pyrophosphate-linked cellobiose functions as an acceptor for the AceA mannosyltransferase reaction in vitro. Neither the chain length nor the saturation state, notably of the first isoprene unit, are critical to the AceA mannosyltransferase activity
-
-
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
show the reaction diagram
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol
show the reaction diagram
-
the enzyme is involved in biosynthesis of xanthan
-
-
?
additional information
additional information
-
-
GumH is involved in biosynthesis of the pentasaccharide repeating unit of xanthan. It is suggested that the wild-type Xanthomonas oryzae-produced xanthan is assembled by the sequential addition of UDP-glucose, UDP-glucose, GDP-mannose, UDPglucuronic acid, and GDP-mannose onto a polyprenol phosphate carrier, by the glycosyltransferase homologues encoded by the gumD, gumM, gumH, gumK, and gumI genes, respectively
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no inhibition by cellobiose
-
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
associated with
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
-
1 * 45000, dynamic light scattering
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 45000, dynamic light scattering
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
maltose-binding-protein-fused enzyme is partially purified by amylose column chromatography, His-tagged enzyme is purified by Ni-NTA column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in fusion with maltose-binding protein in Escherichia coli BL21(DE3) cells and as His-tagged protein in Rosetta(DE3)pLysS cells
-
overproduced in Escherichia coli. The aceA ORF is subcloned into the expression vector pET29 in frame with the S-tag epitope. The recombinant protein is identified, and culture conditions are optimised for production of the soluble protein
-
recombinant mutant AceA expressed in Escherichia coli and in Xanthomonas campestris gumH- strain
-
recombinantly expressed in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E287A
-
mutant enzyme loses activity in vivo and in vitro
E295A
-
mutant enzyme retains residual activity in the in vivo assay
H127A
-
retains reduced but significant activity both in vitro and in vivo
K211A
-
mutant enzyme loses activity in vivo and in vitro
S162A
-
retains reduced but significant activity both in vitro and in vivo
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
method for real-time detection of recombinant GumH enzymatic activity using a Quartz Crystal Microbalance with dissipation monitoring, QCM-D. Monitoring the real-time cellobiose-diphosphate-polyprenol carrier lipid-QCM-D transducer in the presence of GDP-man and GumH enzyme shows a mass increase, indicating the transfer of mannose. The determination of mannosyltransferase function is validated by a HPLC method developed for determination of GDP produced by enzymatic reaction
synthesis
-
the enzyme is involved in biosynthesis of xanthan, an industrially important exopolysaccharide