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Literature summary for 2.4.1.252 extracted from

  • Muniz, J.R.; Alves, C.A.; de Pieri, C.; Beltramini, L.M.; Selistre-de-Araujo, H.S.; Vettore, A.L.; da Silva, F.R.; Arruda, P.; Garratt, R.C.; Oliva, G.; Souza, D.H.
    Overexpression, purification, biochemical characterization, and molecular modeling of recombinant GDP-mannosyltransferase (GumH) from Xylella fastidiosa (2004), Biochem. Biophys. Res. Commun., 315, 485-492.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in fusion with maltose-binding protein in Escherichia coli BL21(DE3) cells and as His-tagged protein in Rosetta(DE3)pLysS cells Xylella fastidiosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
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1 * 45000, dynamic light scattering Xylella fastidiosa

Organism

Organism UniProt Comment Textmining
Xylella fastidiosa
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Purification (Commentary)

Purification (Comment) Organism
maltose-binding-protein-fused enzyme is partially purified by amylose column chromatography, His-tagged enzyme is purified by Ni-NTA column chromatography Xylella fastidiosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-mannose + cellobiose-diphosphate-polyprenol carrier lipid GumH enzyme is an alpha-1,3-mannosyltransferase responsible for the transfer of mannose from GDP-mannose to the cellobiose-diphosphate-polyprenol carrier lipid during the assembly of the tetrasaccharide repeat unit of the exopolysaccharide fastidian gum Xylella fastidiosa GDP + D-mannose-alpha-(1-3)-cellobiose-diphosphate-polyprenol carrier lipid
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?

Subunits

Subunits Comment Organism
monomer 1 * 45000, dynamic light scattering Xylella fastidiosa

Synonyms

Synonyms Comment Organism
GDP-mannosyltransferase
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Xylella fastidiosa
gumH
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Xylella fastidiosa

General Information

General Information Comment Organism
physiological function GumH enzyme is an alpha-1,3-mannosyltransferase responsible for the transfer of mannose from GDP-mannose to the cellobiose-diphosphate-polyprenol carrier lipid during the assembly of the tetrasaccharide repeat unit of the exopolysaccharide fastidian gum Xylella fastidiosa