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Literature summary for 2.4.1.252 extracted from

  • Abdian, P.L.; Lellouch, A.C.; Gautier, C.; Ielpi, L.; Geremia, R.A.
    Identification of essential amino acids in the bacterial (2000), J. Biol. Chem., 275, 40568-40575.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant mutant AceA expressed in Escherichia coli and in Xanthomonas campestris gumH- strain Komagataeibacter xylinus

Protein Variants

Protein Variants Comment Organism
E287A mutant enzyme loses activity in vivo and in vitro Komagataeibacter xylinus
E295A mutant enzyme retains residual activity in the in vivo assay Komagataeibacter xylinus
H127A retains reduced but significant activity both in vitro and in vivo Komagataeibacter xylinus
K211A mutant enzyme loses activity in vivo and in vitro Komagataeibacter xylinus
S162A retains reduced but significant activity both in vitro and in vivo Komagataeibacter xylinus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated with Komagataeibacter xylinus 16020
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Organism

Organism UniProt Comment Textmining
Komagataeibacter xylinus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol AceA is not able to use free cellobiose as a substrate, indicating that the pyrophosphate-lipid moiety is needed for enzymatic activity Komagataeibacter xylinus GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol characterisation of the in vitro reaction product ?
additional information AceA Lys211 and Glu295 are involved in nucleotide sugar donor binding. Glu287 of the EX7E is a potential catalytic residue Komagataeibacter xylinus ?
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Synonyms

Synonyms Comment Organism
alpha-mannosyltransferase AceA
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Komagataeibacter xylinus