Cloned (Comment) | Organism |
---|---|
recombinant mutant AceA expressed in Escherichia coli and in Xanthomonas campestris gumH- strain | Komagataeibacter xylinus |
Protein Variants | Comment | Organism |
---|---|---|
E287A | mutant enzyme loses activity in vivo and in vitro | Komagataeibacter xylinus |
E295A | mutant enzyme retains residual activity in the in vivo assay | Komagataeibacter xylinus |
H127A | retains reduced but significant activity both in vitro and in vivo | Komagataeibacter xylinus |
K211A | mutant enzyme loses activity in vivo and in vitro | Komagataeibacter xylinus |
S162A | retains reduced but significant activity both in vitro and in vivo | Komagataeibacter xylinus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | associated with | Komagataeibacter xylinus | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataeibacter xylinus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol | AceA is not able to use free cellobiose as a substrate, indicating that the pyrophosphate-lipid moiety is needed for enzymatic activity | Komagataeibacter xylinus | GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol | characterisation of the in vitro reaction product | ? | |
additional information | AceA Lys211 and Glu295 are involved in nucleotide sugar donor binding. Glu287 of the EX7E is a potential catalytic residue | Komagataeibacter xylinus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-mannosyltransferase AceA | - |
Komagataeibacter xylinus |