Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is involved in biosynthesis of xanthan, an industrially important exopolysaccharide | Xanthomonas campestris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol | Xanthomonas campestris | the enzyme is involved in biosynthesis of xanthan | GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas campestris | - |
pv. campestris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol | - |
Xanthomonas campestris | GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol | - |
? | |
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol | the enzyme is involved in biosynthesis of xanthan | Xanthomonas campestris | GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gumH | - |
Xanthomonas campestris |
General Information | Comment | Organism |
---|---|---|
malfunction | from the biochemical analysis of a defined set of Xanthomonas campestris gum mutants, experimental data are reported for assigning functions to the products of the gum genes. Inactivation of gumH completely abolishes in vitro polymer formation. Permeabilized cells are incubated with UDP-glucose, GDPmannose, and UDP-glucuronic acid, one of them labeled in the sugar moiety. Accumulating intermediates are analysed. Oligosaccharides obtained from gumH- strain XcH can be resolved into two components: a compound with the same mobility as the disaccharide cellobiose and glucose. XcH cells labeled with UDP-[14C]glucuronic acid or GDP-[14C]mannose do not incorporate radioactivity into the 1203 extract | Xanthomonas campestris |
physiological function | the enzyme is involved in biosynthesis of xanthan | Xanthomonas campestris |