2.7.7.6: DNA-directed RNA polymerase

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For detailed information about DNA-directed RNA polymerase, go to the full flat file.

Reaction

Synonyms

C RNA formation factors, chloroplast soluble RNA polymerase, deoxyribonucleic acid-dependent ribonucleic acid polymerase, DNA-dependent ribonucleate nucleotidyltransferase, DNA-dependent RNA nucleotidyltransferase, DNA-dependent RNA polymerase, DNA-dependent RNA polymerase I, DNA-dependent RNA polymerase III, DNA-dependent RNAP, h-mtRNAP, K1E RNAP, mitochondrial RNA polymerase, mitoRNAP, More, mtRNAP, multi-subunit RNA polymerase, nucleotidyltransferase, ribonucleate, plastid RNA polymerase, plastid-encoded polymerase, plastid-encoded RNA polymerase, Pol I, Pol II, pol III, Pol IIIalpha, Pol IIIbeta, Pol IV, Pol V, polI, PolIII, POLRMT, ribonucleate nucleotidyltransferase, ribonucleate polymerase, ribonucleic acid formation factors, C, ribonucleic acid nucleotidyltransferase, ribonucleic acid polymerase, ribonucleic acid transcriptase, ribonucleic polymerase, ribonucleic transcriptase, rifampicin-resistant RNA polymerase, RNA formation factors, C, RNA nucleotidyltransferase, RNA nucleotidyltransferase (DNA-directed), RNA pol III, RNA polymerase, RNA polymerase core enzyme, RNA polymerase I, RNA polymerase II, RNA polymerase II complex, RNA polymerase III, RNA polymerase III complex, RNA transcriptase, RNAP, RNAP core enzyme, RNAP I, RNAP II, RNAP III, RNAP sigma70, RNAP-II, RNAP2, RNAPII, RPO, rpo1N, Rpo41, RpoA, RpoD, RpoS, RpoT, Saci_0834, sigma38 RNA polymerase, sigmaS-containing RNA polymerase, T7 RNA polymerase, T7 RNAP, T7-like RNA polymerase, Taq polymerase, transcriptase, YonO

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.7 Nucleotidyltransferases

                2.7.7.6 DNA-directed RNA polymerase

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Results	in table
56	Activating Compound
385907	AA Sequence
15	Application
1	CAS Registry Number
77	Cloned(Commentary)
1	Cofactor
34	Crystallization (Commentary)
4851	Disease/ Diagnostics
5	Expression
117	General Information
5	General Stability
8	IC50 Value
164	Inhibitors
44	KM Value [mM]
52	Localization
67	Metals/Ions
122	Molecular Weight [Da]
84	Natural Substrates/ Products (Substrates)
551	Organism
1	Oxidation Stability
4123	PDB ID
29	pH Optimum
3	pH Range
20	Posttranslational Modification
77	Protein Variants
50	Purification (Commentary)
3	Reaction
3	Reaction Type
312	Reference
2	Renatured (Commentary)
67	Source Tissue
26	Specific Activity [micromol/min/mg]
3	Storage Stability
245	Substrates and Products (Substrate)
100	Subunits
403	Synonyms
1	Systematic Name
26	Temperature Optimum [°C]
4	Temperature Range [°C]
8	Temperature Stability [°C]
22	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 2.7.7.6 - DNA-directed RNA polymerase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, 60 mM Tris-HCl buffer, pH 7.8, 20 mM NH4Cl, 1 mM EDTA, 10 mM 2-mercaptoethanol, 40% glycerol, stable for several months

Clostridium acetobutylicum

-

643558

4°C, -20°C or -70°C, 50% w/v glycerol, stable for more than 3 months

Pseudomonas aeruginosa

-

660929

the purified enzyme activity is stable in solution at 4°C even at low ionic strength and in the absence of bivalent metal ions or glycerol, storage in a buffer of low ionic strength containing no bivalent metal ions but 0.001 M EDTA and 10% glycerol at -70°C

Sulfolobus acidocaldarius

-

679628