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2.7.3.3: arginine kinase

This is an abbreviated version!
For detailed information about arginine kinase, go to the full flat file.

Word Map on EC 2.7.3.3

Reaction

ATP
+
L-arginine
=
ADP
 +
Nomega-phospho-L-arginine

Synonyms

adenosine 5'-triphosphate-arginine phosphotransferase, adenosine 5'-triphosphate: L-arginine phosphotransferase, AK, AK-1, AK1, AK2, AK3, AK4, AK: L-arginine phosphotransferase, arginine kinase 1, arginine kinase 2, arginine kinase-1, arginine phosphokinase, ArgK, ARGK-2, ark, ATP: arginine N-phosphotransferase, ATP: arginine phosphotransferase, ATP: L-arginine phosphototransferase, ATP: L-arginine phosphotransferase, ATP:arginine N-phosphotransferase, ATP:arginine phosphotransferase, ATP:L-arginine N-phosphotransferase, ATP:L-arginine omega-N-phosphotransferase, ATP:L-arginine phosphotransferase, ESAK, F32B5.1, F44G3.2, F46H5.3, kinase, arginine (phosphorylating), McsB, MnAK2, MXAN2252 protein, PyAK1, PyAK2, PyAK3, PyAK4, rDer p 20, Tb09.160.4560, Tb927.9.6170, TbAK2, TbAK3, TcAK, TcAK1, TcAK2, W10C8.5, ZC434.8

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.3 Phosphotransferases with a nitrogenous group as acceptor
                2.7.3.3 arginine kinase

Cloned

Cloned on EC 2.7.3.3 - arginine kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AK3 is synthesized using a cell-free protein synthesis system
amplification of cDNA for arginine kinase
amplification of cDNA for arginine kinase. There are three unique arginine kinase genes in the choanoflagellate Monosiga brevicollis
cDNAs of the two-domain arginine kinase and its separated domains 1 and 2 from Anthopleura japonicus, are cloned into the plasmid pMAL, and recombinant enzymes are expressed in Escherichia coli as MBP fusion proteins
cloned in prokaryotic expression plasmid pET-28a, and then expressed in Escherichia coil strain Rosetta in dissoluble form
cloned it in pMAL plasmid and expressed it in Escherichia coli as a fusion protein with maltose-binding protein
cloning of cDNAs into pMAL plasmid and expression in Escherichia coli as a fusion protein with MBP tag or hexameric His tag
DNA and amino acid sequence determination and analysis, sequence comparisons
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21
-
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree
-
domain 2 is separated from the two-domain enzyme and expressed in Escherichia coli, domain 2 still exhibits activity. Expression of mutants of domain 2 in Escherichia coli: H60G, H60R and D197G
-
expressed as a fusion protein with maltose-binding protein in Escherichia coli JM109 cells
-
expressed as a fusion protein with maltose-binding protein in Escherichia coli TB1 cells
expressed as a His-tagged fusion protein in Escherichia coli BL21 cells
expressed as fusion proteins with the maltose-binding protein in Escherichia coli. When expressed alone, domain 1 displays minimal activity. When expressed alone, domain 2 has significantly higher activity and catalytic efficiency that the two-domain wild-type AK, when domain 1 is inactivated using the Y68A mutation, activity is about 50% of the wild-type enzyme and when domain 2 is inactivated using the Y68A mutation, activity is retained at about 12% of the wild-type level
expressed as maltose-binding protein enzyme fusion protein in Escherichia coli TB-1 cells
-
expressed in Escherichia coli
expressed in Escherichia coli as a fusion protein with maltose-binding protein
expressed in Escherichia coli as a fusion with maltose-binding protein
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli BL21 (DE3) codon plus cells by prokaryotic expression plasmid pGEX-4T-2 as glutathione S-transferase arginine kinase fusion protein
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) Codon Plus cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli by two prokaryotic expression plasmids, pET-30a and pET-28a28a. The recombinant protein is expressed as inclusion bodies using pET-30a. Using expression plasmid, pET-28a, and changing the expression conditions results in a soluble and functional form of arginine kinase
expressed in Escherichia coli M15 cells
expressed in Escherichia coli Rosetta cells
expressed in Escherichia coli strain BL21 (DE3)
-
expression in Escherichia coli
expression in Escherichia coli as a histidine-tagged protein
-
expression in Escherichia coli BL21
-
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli BL21(DE3) Rosetta
expression of Cissites arginine kinase protein in Escherichia coli as a fusion with maltose-binding protein
expression of wild-type and mutant enzymes (Y75F, Y75D, P272G, P272R, Y75F/P272G and Y75D/P272R) in Escherichia coli BL21 (DE3)
-
gene AK1 or Tb927.9.6170, located on chromosome 9, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression of full-length and truncated versions of epitope tagged isozyme AK1
gene AK1, DNA and amino acid sequence determination and analysis, recombinant expression of C-terminally His6-tagged isozyme AK1 in Escherichia coli strain BL21 (DE3). The prokaryotic protein expression host Escherichia coli uses the standard genetic code while Tetrahymena pyriformis uses an alternative genetic code where TAA and TAG code for glutamine instead of a stop codon, therefore a TAA codon in AK1 is replaced with CAA
gene AK2, DNA and amino acid sequence determination and analysis, recombinant expression of C-terminally His6-tagged isozyme AK2 in Escherichia coli strain BL21(DE3). The prokaryotic protein expression host Escherichia coli uses the standard genetic code while Tetrahymena pyriformis uses an alternative genetic code where TAA and TAG code for glutamine instead of a stop codon., therefore two TAA and three TAG codons in AK2 are replaced with CAA and CAG
gene argk-1, DNA and amino acid sequence determination and analysis, sequence comparisons, molecular genetic and phylogenetic analysis
gene cloned and inserted into the prokaryotic expression plasmid pET-21b, expression in a soluble and functional form in Escherichia coli
-
gene HcAK, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene Tb09.160.4560, sequence comparisons of Trypanosoma brucei isozymes AK1-3, recombinant expression of N-terminally His-tagged isozyme in Escherichia coli strain BL21(DE3)
gene Tb927.9.6230, sequence comparisons of Trypanosoma brucei isozymes AK1-3
gene TbAK3, sequence comparisons of Trypanosoma brucei isozymes AK1-3
gene TcAK, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene YH65_02995, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
isoform AK2 fused to maltose-binding protein is expressed in Escherichia coli TB1 cells
-
open reading frame of Toxocara canis arginine kinase is cloned into the BamHI/SalI site of pMAL-c2X. The maltose-binding protein (MBP)-Toxocara canis arginine kinase fusion protein is expressed in Escherichia coli TB1 cells by induction with 1 mM IPTG at 25°C for 24 h
-
recombinant expression of His-tagged enzyme in Escherichia coli strain Rossta
-
recombinant expression of His6-tagged isozyme AK1 in Escherichia coli strain TB1
recombinant expression of His6-tagged isozyme AK2 in Escherichia coli strain TB1
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3)
the His6-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells