Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Anthopleura japonica |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the kcat value of the mutant with six Lys residues in the linker region between domains D1 and D2 is reduced to 27.6% that of the two-domain wild-type enzyme | Anthopleura japonica |
Y68G | the mutation in domain 1 or 2 leads to almost no catalytic activity | Anthopleura japonica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
1 * 40000, SDS-PAGE | Anthopleura japonica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anthopleura japonica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Anthopleura japonica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | - |
Anthopleura japonica | ADP + Nomega-phospho-L-Arg | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Anthopleura japonica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
89 | - |
L-Arg | mutant two-domain-enzyme D1(Y68G)-D2, in 100 mM Tris/HCl, pH 8.0 at 25°C | Anthopleura japonica | |
104 | - |
L-Arg | wild type one-domain-enzyme D1, in 100 mM Tris/HCl, pH 8.0 at 25°C | Anthopleura japonica | |
123 | - |
L-Arg | mutant two-domain-enzyme D1-D2(Y68G), in 100 mM Tris/HCl, pH 8.0 at 25°C | Anthopleura japonica | |
157 | - |
L-Arg | wild type one-domain-enzyme D2, in 100 mM Tris/HCl, pH 8.0 at 25°C | Anthopleura japonica | |
187 | - |
L-Arg | mutant two-domain-enzyme D1-Lys6-D2, in 100 mM Tris/HCl, pH 8.0 at 25°C | Anthopleura japonica | |
678 | - |
L-Arg | wild type two-domain-enzyme D1-D2, in 100 mM Tris/HCl, pH 8.0 at 25°C | Anthopleura japonica |