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2.7.3.3: arginine kinase

This is an abbreviated version!
For detailed information about arginine kinase, go to the full flat file.

Word Map on EC 2.7.3.3

Reaction

ATP
+
L-arginine
=
ADP
+
Nomega-phospho-L-arginine

Synonyms

adenosine 5'-triphosphate-arginine phosphotransferase, adenosine 5'-triphosphate: L-arginine phosphotransferase, AK, AK-1, AK1, AK2, AK3, AK4, AK: L-arginine phosphotransferase, arginine kinase 1, arginine kinase 2, arginine kinase-1, arginine phosphokinase, ArgK, ARGK-2, ark, ATP: arginine N-phosphotransferase, ATP: arginine phosphotransferase, ATP: L-arginine phosphototransferase, ATP: L-arginine phosphotransferase, ATP:arginine N-phosphotransferase, ATP:arginine phosphotransferase, ATP:L-arginine N-phosphotransferase, ATP:L-arginine omega-N-phosphotransferase, ATP:L-arginine phosphotransferase, ESAK, F32B5.1, F44G3.2, F46H5.3, kinase, arginine (phosphorylating), McsB, MnAK2, MXAN2252 protein, PyAK1, PyAK2, PyAK3, PyAK4, rDer p 20, Tb09.160.4560, Tb927.9.6170, TbAK2, TbAK3, TcAK, TcAK1, TcAK2, W10C8.5, ZC434.8

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.3 Phosphotransferases with a nitrogenous group as acceptor
                2.7.3.3 arginine kinase

Storage Stability

Storage Stability on EC 2.7.3.3 - arginine kinase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 10 mM Tris-HCl buffer, pH 8.5, activity gradually decreases, but can be restored by addition of 1 mM 2-mercaptoethanol
-80°C, several months
-
0°C, purified enzyme on ice, 24 h, 75% loss of activity
4°C or -10°C, at least 3 months
-
4°C, 3 months, 68% loss of activity
-
4°C, slow loss of activity during prolonged storage, can partially be reactivated by addition of 0.1% w/v 2-mercaptoethanol
-
refrigerated, 80% saturated ammonium sulfate, 20 mM L-Arg, 100 mM 2-mercaptoethanol, pH 7.0. 50% loss of activity in 8-10 days
-