2.7.1.B20: broad specificity nucleoside kinase

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For detailed information about broad specificity nucleoside kinase, go to the full flat file.

Reaction

Synonyms

BTH_I1158, MJ0406, MjNK, NTP:nucleoside kinase, nucleoside kinase, PFK-B sugar kinase, Ta0880

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.B20 broad specificity nucleoside kinase

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Results	in table
3	AA Sequence
3	Cloned(Commentary)
3	Crystallization (Commentary)
2	General Information
2	Inhibitors
14	kcat/KM [mM/s]
18	KM Value [mM]
1	Localization
11	Metals/Ions
6	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
10	Organism
3	pH Optimum
2	pH Range
1	pH Stability
4	Purification (Commentary)
1	Reaction
8	Reference
41	Substrates and Products (Substrate)
6	Subunits
17	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
4	Temperature Stability [°C]
6	Turnover Number [1/s]

Crystallization

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Crystallization on EC 2.7.1.B20 - broad specificity nucleoside kinase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

crystallized as the apoenzyme as well as in complex with an ATP analogue and Mg2+. The latter crystal form is also soaked with D-fructose 6-phosphate. Synchrotron-radiation data are collected to 1.70 A for the apoenzyme crystals and 1.93 A for the complex crystals. All crystals exhibit orthorhombic symmetry. The apoenzyme crystals contain one monomer per asymmetric unit whereas the complex crystals contain a dimer

Methanocaldococcus jannaschii

Q57849

726601

sitting- and hanging-drop vapour diffusion methods, three-dimensional structures of the unliganded enzyme and a complex of the enzyme, an ATP analogue and adenosine are determined to 1.7 and 1.9 A resolution, respectively. In the crystal structure of the MjNK complex, subunit A adopts a closed conformation and subunit B an open conformation. In subunit A all substrates and Mg2+ are observed, whereas in subunit B only the ATP analogue can be clearly identified in the electron density

Methanocaldococcus jannaschii

Q57849

726575

sitting nanodroplet vapor diffusion method, crystal structure of Ta0880, determined at 1.91 A resolution reveals a dimer with each monomer composed of an alpha/beta/alpha sandwich domain and a smaller lid domain

Thermoplasma acidophilum

Q9HJT3

728780