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Literature summary for 2.7.1.B20 extracted from
- A broad specificity nucleoside kinase from Thermoplasma acidophilum (2013), Proteins, 81, 568-582.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Thermoplasma acidophilum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting nanodroplet vapor diffusion method, crystal structure of Ta0880, determined at 1.91 A resolution reveals a dimer with each monomer composed of an alpha/beta/alpha sandwich domain and a smaller lid domain | Thermoplasma acidophilum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000208 | - |
guanosine | pH 8.0, 22°C | Thermoplasma acidophilum |  |
| 0.000411 | - |
cytidine | pH 8.0, 22°C | Thermoplasma acidophilum | |
| 0.000712 | - |
Inosine | pH 8.0, 22°C | Thermoplasma acidophilum | |
| 0.001 | - |
adenosine | pH 8.0, 22°C | Thermoplasma acidophilum | |
| 0.0469 | - |
ATP | pH 8.0, 22°C, cosubstrate: inosine | Thermoplasma acidophilum | |
| 0.185 | - |
GTP | pH 8.0, 22°C | Thermoplasma acidophilum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Thermoplasma acidophilum | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | kinase activity is observed with Mg2+ and Co2+ and to a lesser degree with Mn2+ | Thermoplasma acidophilum | |
| Mg2+ | kinase activity is observed with Mg2+ and Co2+ and to a lesser degree with Mn2+ | Thermoplasma acidophilum | |
| Mn2+ | kinase activity is observed with Mg2+ and Co2+ and to a lesser degree with Mn2+ | Thermoplasma acidophilum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32804 | - |
2 * 32804, calculated from sequence | Thermoplasma acidophilum |
| 33000 | - |
2 * 33000, SDS-PAGE, crystal structure | Thermoplasma acidophilum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoplasma acidophilum | Q9HJT3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoplasma acidophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + adenosine | catalytic efficiency with cytidine is 22% relative to guanosine | Thermoplasma acidophilum | ADP + adenosine 5'-phosphate | - |
? | |
| ATP + cytidine | catalytic efficiency with cytidine is 45% relative to guanosine | Thermoplasma acidophilum | ADP + cytidine 5'-phosphate | - |
? | |
| ATP + guanosine | phosphoryl donor specificity in order of decreasing Vmax: GTP > ATP > ITP. The catalytic efficiency of GTP is 39% compared to that of ATP | Thermoplasma acidophilum | ADP + guanosine 5'-phosphate | - |
? | |
| ATP + inosine | catalytic efficiency with inosine is 62% relative to guanosine | Thermoplasma acidophilum | ADP + inosine 5'-phosphate | - |
? | |
| GTP + guanosine | phosphoryl donor specificity in order of decreasing Vmax: GTP > ATP > ITP. The catalytic efficiency of GTP is 39% compared to that of ATP | Thermoplasma acidophilum | GDP + guanosine 5'-phosphate | - |
? | |
| ITP + guanosine | phosphoryl donor specificity in order of decreasing Vmax: GTP > ATP > ITP | Thermoplasma acidophilum | IDP + guanosine 5'-phosphate | - |
? | |
| additional information | negligible enzymatic activity with thymidine, uridine, and 2-deoxyadenosine. Negligible enzymatic activity is observed with UTP and TTP as phosphate donors | Thermoplasma acidophilum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 32804, calculated from sequence | Thermoplasma acidophilum |
| dimer | 2 * 33000, SDS-PAGE, crystal structure | Thermoplasma acidophilum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ta0880 | locus name | Thermoplasma acidophilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Thermoplasma acidophilum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.064 | - |
cytidine | pH 8.0, 22°C | Thermoplasma acidophilum | |
| 0.072 | - |
guanosine | pH 8.0, 22°C | Thermoplasma acidophilum | |
| 0.084 | - |
adenosine | pH 8.0, 22°C | Thermoplasma acidophilum | |
| 0.16 | - |
Inosine | pH 8.0, 22°C | Thermoplasma acidophilum | |
| 0.17 | - |
ATP | pH 8.0, 22°C, cosubstrate: inosine | Thermoplasma acidophilum | |
| 0.27 | - |
GTP | pH 8.0, 22°C | Thermoplasma acidophilum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
- |
Thermoplasma acidophilum |
| 8 | - |
assay at | Thermoplasma acidophilum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 7.8 | pH 6.2: about 60% of maximal activity, pH 7.8: about 75% of maximal activity | Thermoplasma acidophilum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
GTP | pH 8.0, 22°C | Thermoplasma acidophilum | |
| 3.7 | - |
ATP | pH 8.0, 22°C, cosubstrate: inosine | Thermoplasma acidophilum | |
| 74.9 | - |
adenosine | pH 8.0, 22°C | Thermoplasma acidophilum | |
| 156 | - |
cytidine | pH 8.0, 22°C | Thermoplasma acidophilum | |
| 215 | - |
Inosine | pH 8.0, 22°C | Thermoplasma acidophilum | |
| 345 | - |
guanosine | pH 8.0, 22°C | Thermoplasma acidophilum | |
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