2.7.1.182: phytol kinase
This is an abbreviated version!
For detailed information about phytol kinase, go to the full flat file.
Word Map on EC 2.7.1.182
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2.7.1.182
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chlorophyll
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tocopherol
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geranylgeranyl
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chlorophyllide
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geranylgeranyl-diphosphate
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photosystem
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tomato
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phytyl
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synechocystis
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prenyl
- 2.7.1.182
- chlorophyll
- tocopherol
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geranylgeranyl
- chlorophyllide
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geranylgeranyl-diphosphate
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photosystem
- tomato
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phytyl
- synechocystis
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prenyl
Reaction
Synonyms
At5g04490, phytol kinase, SlVTE5, VTE, VTE5
ECTree
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Engineering
Engineering on EC 2.7.1.182 - phytol kinase
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additional information
construction of an independent mutant allele (pst12490, vte5-2) that carries a transposon insertion in the first exon behind the start codon of the VTE5 gene and shows no vte5 expression and absence of VTE5 mRNA. Construction of VTE5/VTE6 double mutant plants, which are tocopherol-deficient and contain more chlorophyll, but reduced amounts of phytol and phytyl-phosphate compared with vte6 mutants, suggesting that phytol or phytyl-phosphate are detrimental to plant growth. The growth defect of vte6-1 is alleviated in the vte5-2/vte6-1 double mutant. The tocopherol-deficient mutants vte1, vte2, vte4, and vte5 can grow on soil and produce fertile seeds, while vte6-1 and vte6-2 cannot grow photoautotrophically and are infertile. Amounts of phytol are unchanged in vte5-2 and vte5-2/vte6-1 mutants compared with the wild-type, the different forms of fatty acid phytyl esters are increased in leaves of vte5-2, vte6-1, vte6-2, and vte5-2 vte6-1 compared with the wild type, especially 12:0-phytol, 14:0-phytol, and 16:0-phytol
additional information
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construction of an independent mutant allele (pst12490, vte5-2) that carries a transposon insertion in the first exon behind the start codon of the VTE5 gene and shows no vte5 expression and absence of VTE5 mRNA. Construction of VTE5/VTE6 double mutant plants, which are tocopherol-deficient and contain more chlorophyll, but reduced amounts of phytol and phytyl-phosphate compared with vte6 mutants, suggesting that phytol or phytyl-phosphate are detrimental to plant growth. The growth defect of vte6-1 is alleviated in the vte5-2/vte6-1 double mutant. The tocopherol-deficient mutants vte1, vte2, vte4, and vte5 can grow on soil and produce fertile seeds, while vte6-1 and vte6-2 cannot grow photoautotrophically and are infertile. Amounts of phytol are unchanged in vte5-2 and vte5-2/vte6-1 mutants compared with the wild-type, the different forms of fatty acid phytyl esters are increased in leaves of vte5-2, vte6-1, vte6-2, and vte5-2 vte6-1 compared with the wild type, especially 12:0-phytol, 14:0-phytol, and 16:0-phytol
additional information
generation of SlVTE5-RNAi transgenic lines, RNAi-mediated silencing of the Solyc03g071720 locus using primers RNAi-VTE5-F and RNAi-VTE5-R. TILLING-based molecular screening is used to identify a loss-offunction mutation in SlFOLK using an EMS-mutagenized tomato collection
additional information
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generation of SlVTE5-RNAi transgenic lines, RNAi-mediated silencing of the Solyc03g071720 locus using primers RNAi-VTE5-F and RNAi-VTE5-R. TILLING-based molecular screening is used to identify a loss-offunction mutation in SlFOLK using an EMS-mutagenized tomato collection
additional information
generation of an RNAi line (vte5) from cv. Micro-Tom with a downregulation of 76% in under control conditions and reduced levels of alpha-tocopherol. Mutant vte5 has plastoquinone levels similar to those of the wild-type under combined stress, the strong phenotype can be attributed to the lack of alpha-tocopherol. Mutant vte5 plants accumulate free phytol in the leaf. Phenotype, overview
additional information
-
generation of an RNAi line (vte5) from cv. Micro-Tom with a downregulation of 76% in under control conditions and reduced levels of alpha-tocopherol. Mutant vte5 has plastoquinone levels similar to those of the wild-type under combined stress, the strong phenotype can be attributed to the lack of alpha-tocopherol. Mutant vte5 plants accumulate free phytol in the leaf. Phenotype, overview