Cloned (Comment) | Organism |
---|---|
gene VTE5, recombinant functional expression of gene VTE5 or functional coexpression of genes VTE5 and VTE6 in Escherichia coli, quantitative real-time PCR expression analysis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an independent mutant allele (pst12490, vte5-2) that carries a transposon insertion in the first exon behind the start codon of the VTE5 gene and shows no vte5 expression and absence of VTE5 mRNA. Construction of VTE5/VTE6 double mutant plants, which are tocopherol-deficient and contain more chlorophyll, but reduced amounts of phytol and phytyl-phosphate compared with vte6 mutants, suggesting that phytol or phytyl-phosphate are detrimental to plant growth. The growth defect of vte6-1 is alleviated in the vte5-2/vte6-1 double mutant. The tocopherol-deficient mutants vte1, vte2, vte4, and vte5 can grow on soil and produce fertile seeds, while vte6-1 and vte6-2 cannot grow photoautotrophically and are infertile. Amounts of phytol are unchanged in vte5-2 and vte5-2/vte6-1 mutants compared with the wild-type, the different forms of fatty acid phytyl esters are increased in leaves of vte5-2, vte6-1, vte6-2, and vte5-2 vte6-1 compared with the wild type, especially 12:0-phytol, 14:0-phytol, and 16:0-phytol | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + phytol | Arabidopsis thaliana | - |
CDP + phytyl phosphate | phytyl phosphate is phosphorylated by phytyl phosphate kinase, VTE6 | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9LZ76 | gene VTE5 or At5g04490 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
seed | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + phytol | - |
Arabidopsis thaliana | CDP + phytyl phosphate | phytyl phosphate is phosphorylated by phytyl phosphate kinase, VTE6 | ? |
Synonyms | Comment | Organism |
---|---|---|
VTE5 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CTP | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | the growth defect of vte6-1 is alleviated in the vte5-2/vte6-1 double mutant. The tocopherol-deficient mutants vte1, vte2, vte4, and vte5 can grow on soil and produce fertile seeds, while vte6-1 and vte6-2 cannot grow photoautotrophically and are infertile. Amounts of phytol are unchanged in vte5-2 and vte5-2/vte6-1 mutants compared with the wild-type | Arabidopsis thaliana |
metabolism | phytol from chlorophyll degradation can be phosphorylated to phytyl-phosphate by phytol kinase, EC 2.7.1.182, and to phytyl-diphosphate, the substrate for tocopherol (vitamin E) synthesis by phytyl-diphosphate kinase, EC 2.7.4.BX. Tocopherol synthesis in leaves depends on phytol derived from chlorophyll, not on de novo synthesis of phytyl-diphosphate from geranylgeranyl-diphosphate. The phytol phosphorylation pathway is essential for plant growth and development | Arabidopsis thaliana |
physiological function | the essential enzyme is required for tocopherol synthesis and photoautotrophic growth synthesizing phytyl phosphate from phytol from degenerated chlorophyll in the phytol phosphorylation pathway | Arabidopsis thaliana |