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2.7.1.175: maltokinase

This is an abbreviated version!
For detailed information about maltokinase, go to the full flat file.

Word Map on EC 2.7.1.175

Reaction

ATP
+
maltose
=
ADP
+
alpha-maltose 1-phosphate

Synonyms

ATP:maltose 1-phosphotransferase, Mak, Mak1, maltose-1-phosphate synthetase, Msm Pep2, Pep2

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.175 maltokinase

Engineering

Engineering on EC 2.7.1.175 - maltokinase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D321A
site-directed mutagenesis, the Mg2+ binding residue mutant is inactive
D339N
inactive mutant
E340R
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
K145A
site-directed mutagenesis, the Mg2+ binding residue mutant is inactive
N145A
site-directed mutagenesis, inactive mutant
Q309A
site-directed mutagenesis, the Mg2+ binding residue mutant is inactive
R351A
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
S144A
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme
D321A
-
site-directed mutagenesis, the Mg2+ binding residue mutant is inactive
-
D339N
-
inactive mutant
-
E340R
-
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
-
K145A
-
site-directed mutagenesis, the Mg2+ binding residue mutant is inactive
-
N145A
-
site-directed mutagenesis, inactive mutant
-
Q309A
-
site-directed mutagenesis, the Mg2+ binding residue mutant is inactive
-
R351A
-
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
-
S144A
-
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme
-
E324R
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
K413A
site-directed mutagenesis
N137A
site-directed mutagenesis, inactive mutant
R334R
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
S136A
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme
Y416A
site-directed mutagenesis
Y416F
site-directed mutagenesis
Y420A
site-directed mutagenesis
Y420F
site-directed mutagenesis
N137A
-
site-directed mutagenesis, inactive mutant
-
S136A
-
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme
-
Y416A
-
site-directed mutagenesis
-
Y420A
-
site-directed mutagenesis
-
Y420F
-
site-directed mutagenesis
-